GENERATION OF ANALYTIC PLASMA-LIPOPROTEIN PROFILES USING 2 PREPACKED SUPEROSE-6B COLUMNS
GENERATION OF ANALYTIC PLASMA-LIPOPROTEIN PROFILES USING 2 PREPACKED SUPEROSE-6B COLUMNS
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DOI：
10.1016/0009-8981(92)90348-t
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发表时间：
1992-01-31
影响因子：
5
通讯作者：
FAERGEMAN, O
中科院分区：
文献类型：
--
作者：
GERDES, LU;GERDES, C;FAERGEMAN, O
一个简单的，廉价的色谱系统，从总的人血浆中产生血浆脂蛋白谱进行了测试，血浆中的血脂正常的受试者和杂合子家族性高胆固醇血症，高α-脂蛋白血症，脂蛋白脂肪酶缺乏症，家族性β-脂蛋白异常血症和家族性卵磷脂胆固醇酰基转移酶缺乏症的患者。该系统似乎是一个很好的替代更昂贵的高压液相色谱系统，特别是在脂蛋白实验室已经提供了柱色谱设备。如果实验室中有微量板光度计和计算机，则可以简化柱中70-80个洗脱液级分中各种脂质的测量。
A simple, low-priced chromatographic system to generate plasma lipoprotein profiles from total human plasma was tested with plasma from normolipidemic subjects and patients with heterozygous familial hypercholesterolemia, hyper-alpha-lipoproteinemia, lipoprotein lipase deficiency, familial dysbetalipoproteinemia and familial lecithin-cholesterol-acyl-transferase deficiency. The system appears to be a good alternative to more expensive high-pressure liquid chromatography systems, notably in lipoprotein laboratories already provided with equipment for column chromatography. If a microplate photometer and a computer is available in the laboratory, the measurement of various lipids in 70-80 eluant fractions from the columns can be simplified.