Effectable application of vascular endothelial growth factor to critical sized rat calvaria defects
Effectable application of vascular endothelial growth factor to critical sized rat calvaria defects
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DOI：
10.1016/j.tripleo.2009.09.010
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发表时间：
2010-02-01
期刊：
影响因子：
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通讯作者：
Noguchi, Kazuyuki
中科院分区：
文献类型：
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作者：
Yonamine, Yutaka;Matsuyama, Takashi;Noguchi, Kazuyuki
Objective.血管生成的早期血管反应对于骨缺损愈合的正常进展是必不可少的。血管内皮生长因子（VEGF）是一种强有力的血管生成诱导剂。本研究的目的是评估聚（L，D-乳酸-共-乙醇酸）（PLGA）膜与VEGF封装到PLGA微球对大鼠颅骨骨缺损处骨再生的影响。制备了包含VEGF 165的PLGA微球（VEGF微球），并在大鼠颅骨中产生临界尺寸的骨缺损。将VEGF微球、PLGA微球或VEGF微球加PLGA膜应用于缺损。通过软X线片和组织学检查，使用图像分析评价骨再生。与应用其他治疗方法的骨缺损部位相比，应用VEGF微球/PLGA膜的骨缺损部位观察到成熟的厚骨再生。结论。VEGF微球和PLGA膜的组合有效地增强骨再生。（口腔外科口腔医学口腔病理学口腔放射学Endod 2010; 109：225-231）
Objective. An early vascular response for angiogenesis is essential for the normal progression of bone defect healing. Vascular endothelial growth factor ( VEGF) is a potent inducer of angiogenesis. The aim of this study was to evaluate the effects of a poly ( L, D-lactic-co-glycolic acid) ( PLGA) membrane with VEGF encapsulated into PLGA microspheres on bone regeneration at bone defects in rat calvaria.Study Design. Microspheres of PLGA incorporating VEGF165 ( VEGF microspheres) were prepared, and critical-size bone defects were created in rat calvaria. The VEGF microspheres, PLGA microspheres, or VEGF microspheres plus PLGA membrane were applied to the defects. Bone regeneration was evaluated using image analysis based on soft radiographic and histologic examination.Results. Mature thick bone regeneration was observed in selected sites at bone defects that had been applied with VEGF microspheres/PLGA membrane compared with those that had been applied with the other treatments.Conclusion. A combination of VEGF microspheres and a PLGA membrane effectively enhances bone regeneration. ( Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010; 109: 225-231)