A colorimetric/fluorescence dual-channel probe for highly discriminating detection of cysteine
A colorimetric/fluorescence dual-channel probe for highly discriminating detection of cysteine
复制标题
用于高度区分检测半胱氨酸的比色/荧光双通道探针
DOI：
10.1016/j.talanta.2018.11.007
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发表时间：
2019
期刊：
影响因子：
6.1
通讯作者：
Liang Xiu Xia
中科院分区：
文献类型：
--
作者：
Li Kai Bin;Qu Wang Bo;Han De Man;Zhang Siqi;Shi Wei;Chen Cai Xia;Liang Xiu Xia
在这项工作中，报道了一种新型的荧光探针，用于选择性和灵敏地检测具有比色和FL双重信号变化的半胱氨酸。该探针是由磺酰基苯并恶二唑(SBD)染料与含有刚性哌嗪基团的丹磺酰氯反应两步偶联而成。该探针在含有纳米分子LOD的水溶液中显示出对半胱氨酸的特异性关闭-开启响应，并且不受一系列氨基酸和几种竞争分析物的干扰。当加入半胱氨酸后，该探针将经过连续的取代反应和分子内重排反应，生成4-氨基丁二烯衍生物，导致在575 nm处产生强烈的黄色荧光发射，并伴随着吸收光谱的两步红移。此外，它还可用于活体细胞内源性半胱氨酸的成像。
In this work, a novel fluorescence (FL) probe for selective and sensitive detection of Cys with colorimetric and FL dual signal changes was reported. The probe was synthesized by two step of sulfonamide reaction coupling between a sulfonyl benzoxadiazole (SBD) dye and dansyl chloride linked with rigid piperazine group. The probe showed a specific off-on response to Cys in aqueous solution with nanomolar LOD, and without interference by a range of amino acids and several competing analytes. Upon addition of Cys, the probe will undergo sequential substitution and intramolecular rearrangement reactions, yielding a 4-amino SBD derivative, which results in generation of strong yellow fluorescence emission at 575 nm accompanied by a two-step red shift in the absorption spectral. Moreover, it can be used for imaging of endogenous Cys in living cells.