GPD1, which encodes glycerol-3-phosphate dehydrogenase, is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by the high-osmolarity glycerol response pathway
GPD1, which encodes glycerol-3-phosphate dehydrogenase, is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by the high-osmolarity glycerol response pathway
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DOI：
10.1128/mcb.14.6.4135-4144.1994
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发表时间：
1994-06
影响因子：
5.3
通讯作者：
J. Albertyn;S. Hohmann;J. Thevelein;B. Prior
中科院分区：
文献类型：
--
作者：
J. Albertyn;S. Hohmann;J. Thevelein;B. Prior
酵母酿酒酵母对渗透胁迫有反应，即，通过甘油作为相容性溶质的产量增加和细胞内积累，增加生长培养基的渗透压。我们克隆了一个编码甘油合成关键酶的基因，即依赖于NADH的胞浆甘油-3-磷酸脱氢酶，并将其命名为GPD 1。GPD 1 δ突变体产生非常少的甘油，并且它们对渗透胁迫敏感。因此，甘油生产确实是必要的酵母细胞的生长过程中减少水的可用性。当施加渗透胁迫时，缺乏参与渗透胁迫诱导的信号转导（高渗透压甘油反应[HOG]途径）的蛋白激酶的hog 1 δ突变体未能增加甘油-3-磷酸脱氢酶活性和mRNA水平。因此，GPD 1的表达通过HOG途径调节。然而，有可能是Hog 1独立的机制介导的胁迫诱导的甘油积累，因为hog 1 δ菌株仍然可以提高其甘油含量，虽然低于野生型。HOG 1 δ突变体比同基因GPD 1 δ菌株对渗透胁迫更敏感，并且GPD 1 δ HOG 1 δ双突变体甚至比任一单突变体更敏感。因此，HOG通路最有可能在适应高渗介质的机制中具有额外的靶点。
The yeast Saccharomyces cerevisiae responds to osmotic stress, i.e., an increase in osmolarity of the growth medium, by enhanced production and intracellular accumulation of glycerol as a compatible solute. We have cloned a gene encoding the key enzyme of glycerol synthesis, the NADH-dependent cytosolic glycerol-3-phosphate dehydrogenase, and we named it GPD1. gpd1 delta mutants produced very little glycerol, and they were sensitive to osmotic stress. Thus, glycerol production is indeed essential for the growth of yeast cells during reduced water availability. hog1 delta mutants lacking a protein kinase involved in osmostress-induced signal transduction (the high-osmolarity glycerol response [HOG] pathway) failed to increase glycerol-3-phosphate dehydrogenase activity and mRNA levels when osmotic stress was imposed. Thus, expression of GPD1 is regulated through the HOG pathway. However, there may be Hog1-independent mechanisms mediating osmostress-induced glycerol accumulation, since a hog1 delta strain could still enhance its glycerol content, although less than the wild type. hog1 delta mutants are more sensitive to osmotic stress than isogenic gpd1 delta strains, and gpd1 delta hog1 delta double mutants are even more sensitive than either single mutant. Thus, the HOG pathway most probably has additional targets in the mechanism of adaptation to hypertonic medium.