Proteomic and metabolomic analysis reveal gender-specific responses of mussel Mytilus galloprovincialis to 2,2′,4,4′-tetrabromodiphenyl ether (BDE 47)
Proteomic and metabolomic analysis reveal gender-specific responses of mussel Mytilus galloprovincialis to 2,2′,4,4′-tetrabromodiphenyl ether (BDE 47)
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DOI：
10.1016/j.aquatox.2013.07.009
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发表时间：
2013-09-15
影响因子：
4.5
通讯作者：
Yu, Junbao
中科院分区：
文献类型：
--
作者：
Ji, Chenglong;Wu, Huifeng;Yu, Junbao
多溴联苯醚(PBDEs)是一类广泛应用于工业产品中的溴化阻燃剂，对中国莱州湾沿岸环境构成了潜在的危险。由于它们的毒性，如肝脏毒性、致癌性、神经毒性、免疫毒性和对动物的内分泌干扰作用，引起了人们的极大关注。在这项工作中，我们使用蛋白质组学和代谢组学相结合的方法，重点研究了贝类中BDE47的性别特异性反应。代谢反应表明，BDE47主要引起雄性贻贝鳃能量代谢紊乱。对于雌性贻贝样品，渗透调节和能量代谢都受到干扰，表现为不同的代谢特征。蛋白质组学研究表明，BDE-47可诱导雄性和雌性贻贝细胞凋亡，降低其体内的活性氧(ROS)生成，雄性贻贝体内的蛋白质动态平衡受到干扰，雌性贻贝的蛋白质降解作用受到干扰，这是基于蛋白质组学上的差异。总体而言，这些结果证实了贻贝对OD47暴露的性别特异性反应。这项工作表明，代谢组学和蛋白质组学相结合的方法可以为环境污染物对生物体的毒理学影响提供重要的见解。(C)2013爱思唯尔B.V.保留所有权利。
Polybrominated diphenyl ethers (PBDEs) are a class of brominated flame-retardants (BFRs) that are widely used in industrial products and have posed potential risk on the coastal environment of the Laizhou Bay in China. They are of great concern due to their toxicities, such as hepatotoxicity, carcinogenecity, neurotoxicity, immunotoxicity and endocrine disrupting effects in animals. In this work, we focused on the gender-specific responses of BDE 47 in mussel Mytilus galloprovincialis using a combined proteomic and metabolomic approach. Metabolic responses indicated that BDE 47 mainly caused disturbance in energy metabolism in male mussel gills. For female mussel samples, disruption in both osmotic regulation and energy metabolism was found in terms of differential metabolic profiles. Proteomic responses revealed that BDE 47 induced cell apoptosis and reduced reactive oxygen species (ROS) production in both male and female mussels, disturbance in protein homeostasis in male mussels as well as disturbance in female mussel proteolysis based on the differential proteomic biomarkers. Overall, these results confirmed the gender-specific responses in mussels to ODE 47 exposures. This work demonstrated that an integrated metabolomic and proteomic approach could provide an important insight into the toxicological effects of environmental pollutant to organisms. (C) 2013 Elsevier B.V. All rights reserved.