Use of gel filtration in the preparation of biological fluids for magnetic resonance spectroscopy.
Use of gel filtration in the preparation of biological fluids for magnetic resonance spectroscopy.
复制标题
凝胶过滤在制备用于磁共振波谱的生物液体中的应用。
DOI：
10.1002/mrm.1910130319
复制
发表时间：
1990
影响因子：
3.3
通讯作者：
Spicer,LD
中科院分区：
文献类型：
--
作者：
Hoffman,DW;Venters,RA;Shedd,SF;Spicer,LD
通过质子磁共振光谱分析生物流体通常由于大的水共振产生的动态范围问题而变得复杂。凝胶过滤色谱法被发现是一种简单且无损的方法，用于将D2O交换为H2O，并用于从血浆和尿液中去除低分子量分子，显着改善随后的一维和二维MRS光谱。
Analysis of biological fluids by proton magnetic resonance spectroscopy is often complicated by dynamic range problems created from the large water resonance. Gel filtration chromatography is found to be a simple and nondestructive method for exchanging D2O for H2O and for removing low molecular weight molecules from both plasma and urine, significantly improving subsequent one‐ and two‐dimensional MRS spectra.