Continuous dielectrophoretic cell separation microfluidic device
Continuous dielectrophoretic cell separation microfluidic device
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DOI：
10.1039/b613344d
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发表时间：
2007-01-01
期刊：
影响因子：
6.1
通讯作者：
Kaler, Karan V. I. S.
中科院分区：
文献类型：
--
作者：
Li, Youlan;Dalton, Colin;Kaler, Karan V. I. S.
我们提出了一种用于生物细胞样品悬浮液的连续介电泳法(DEP)分离和纯化的原型微流控装置。该装置集成了三个功能齐全的不同单元，包括一个进样器、一个分馏区和两个出口。在鞘和进样口，细胞样品被流体力学聚焦成宽度可控的流；在DEP分级区，合成并使用特殊形状的非均匀(等向运动)电场来促进分离，然后将分类后的细胞输送到两个样品采集口。模拟了喷嘴区域的微流体行为，并进行了实验验证。以酵母细胞为模型生物颗粒，对该装置的运行和性能进行了评价。详细讨论了与该器件的制造和操作有关的问题。这种设备朝着集成的芯片实验室设备迈出了重要的一步，该设备可以与许多其他芯片上的组件接口/集成，以便该设备承担整个实验室程序。
We present a prototype microfluidic device developed for the continuous dielectrophoretic (DEP) fractionation and purification of sample suspensions of biological cells. The device integrates three fully functional and distinct units consisting of an injector, a fractionation region, and two outlets. In the sheath and sample injection ports, the cell sample are hydrodynamically focused into a stream of controlled width; in the DEP fractionation region, a specially shaped nonuniform (isomotive) electric field is synthesized and employed to facilitate the separation, and the sorted cells are then delivered to two sample collection ports. The microfluidic behavior of the injector region was simulated and then experimentally verified. The operation and performance of the device was evaluated using yeast cells as model biological particles. Issues relating to the fabrication and operation of the device are discussed in detail. Such a device takes a significant step towards an integrated lab-on-a-chip device, which could interface/integrate to a number of other on-chip components for the device to undertake the whole laboratory procedure.