SPT4, SPT5 and SPT6 interactions: effects on transcription and viability in Saccharomyces cerevisiae.
SPT4, SPT5 and SPT6 interactions: effects on transcription and viability in Saccharomyces cerevisiae.
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SPT4、SPT5 和 SPT6 相互作用：对酿酒酵母转录和活力的影响。
DOI：
10.1093/genetics/132.2.325
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发表时间：
1992
期刊：
影响因子：
3.3
通讯作者：
Winston,F
中科院分区：
文献类型：
--
作者：
Swanson,MS;Winston,F
酿酒酵母的 SPT4、SPT5 和 SPT6 基因最初是通过抑制 HIS4 和 LYS2 处 delta 插入突变的突变来鉴定的。随后的分析表明，spt4、spt5 和 spt6 突变赋予相似的多效性表型。它们通过改变转录来抑制δ插入突变，并被认为是其他几个基因座正常转录所必需的。我们现在已经分析了 SPT4、SPT5 和 SPT6 之间的相互作用。首先，这三个基因中任意两个的突变组合会导致单倍体致死。其次，该组不同成员中的一些隐性突变无法相互补充。第三，所有三个基因的突变都以类似的方式改变转录。最后，免疫共沉淀实验的结果证明至少SPT5和SPT6蛋白存在物理相互作用。总而言之，这些遗传和生化结果表明，SPT4、SPT5 和 SPT6 在转录过程中共同发挥作用，这对于酵母的生存至关重要。
The SPT4, SPT5 and SPT6 genes of Saccharomyces cerevisiae were identified originally by mutations that suppress delta insertion mutations at HIS4 and LYS2. Subsequent analysis has demonstrated that spt4, spt5 and spt6 mutations confer similar pleiotropic phenotypes. They suppress delta insertion mutations by altering transcription and are believed to be required for normal transcription of several other loci. We have now analyzed interactions between SPT4, SPT5 and SPT6. First, the combination of mutations in any two of these three genes causes lethality in haploids. Second, some recessive mutations in different members of this set fail to complement each other. Third, mutations in all three genes alter transcription in similar ways. Finally, the results of coimmunoprecipitation experiments demonstrate that at least the SPT5 and SPT6 proteins interact physically. Taken together, these genetic and biochemical results indicate that SPT4, SPT5 and SPT6 function together in a transcriptional process that is essential for viability in yeast.