EFFECTS OF ACIDIC FIBROBLAST GROWTH-FACTOR AND EPIDERMAL GROWTH-FACTOR ON SUBCONFLUENT FETAL-RAT CALVARIA CELL-CULTURES - DNA-SYNTHESIS AND ALKALINE-PHOSPHATASE ACTIVITY
EFFECTS OF ACIDIC FIBROBLAST GROWTH-FACTOR AND EPIDERMAL GROWTH-FACTOR ON SUBCONFLUENT FETAL-RAT CALVARIA CELL-CULTURES - DNA-SYNTHESIS AND ALKALINE-PHOSPHATASE ACTIVITY
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DOI：
10.1016/0169-6009(90)90117-x
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发表时间：
1990-02-01
期刊：
影响因子：
--
通讯作者：
FOREST, N
中科院分区：
文献类型：
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作者：
NICOLAS, V;NEFUSSI, JR;FOREST, N
酸性成纤维细胞生长因子（aFGF）和表皮生长因子（EGF）的影响进行了检查，在亚融合胎鼠颅骨细胞培养，在2%的血清的存在下。aFGF和EGF对DNA合成的最大作用在18小时后观察到，所述DNA合成通过[3 H]胸苷掺入来测量。aFGF刺激DNA合成3.5倍，ED 50为0.75 ng/ml，而EGF刺激2.3倍，ED 50为0.067 ng/ml。5-溴-2-脱氧尿苷染色显示散在细胞中的增殖刺激高于细胞簇。18小时aFGF或EGF治疗碱性磷酸酶（ALP）活性分别下降40%和23%，与对照培养物相比。这种抑制作用在效应物存在下48 h后更为明显，但未观察到ALP电泳迁移率的改变。这些数据表明，aFGF是一个较弱的有丝分裂原比EGF和更高的抑制剂的ALP活性在胎鼠颅骨细胞培养。
The effects of acidic fibroblast growth factor (aFGF) and epidermal growth factor (EGF) were examined in subconfluent fetal rat calvaria cell cultures, in the presence of 2% serum. Maximal effect of aFGF and EGF on DNA synthesis measured by [3H]thymidine incorporation was observed after 18h. aFGF stimulated DNA synthesis by 3.5-fold with an ED50 of 0.75 ng/ml while a 2.3-fold EGF stimulation was recorded with an ED50 of 0.067 ng/ml. 5-Bromo-2-deoxyuridine staining showed a higher stimulation of proliferation in the scattered cells than in the cell clusters. An 18 h aFGF or EGF treatment decreased alkaline phosphatase (ALP) activity by 40 and 23%, respectively, as compared with control cultures. This inhibition was more pronounced after 48 h in the presence of effectors but no modification of the ALP electrophoretic mobility was observed. These data suggest that aFGF is a less potent mitogen than EGF and a higher inhibitor of ALP activity in fetal rat calvaria cell culture.