Specific association of Type I c-Abl with Ran GTPase in lipopolysaccharide-mediated differentiation.
Specific association of Type I c-Abl with Ran GTPase in lipopolysaccharide-mediated differentiation.
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I 型 c-Abl 与 Ran GTPase 在脂多糖介导的分化中的特异性关联。
DOI：
10.1038/sj.onc.1204361
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Wong,PM
中科院分区：
文献类型：
--
作者：
Daniel,R;Chung,SW;Eisenstein,TK;Sultzer,BM;Wong,PM
c-Abl 的几种亚型中的每一种都可能涉及不同的生物学功能。 I 型 c-Abl 已被证明参与 LPS 诱导的分化和 IV 型 c-Abl 细胞凋亡。 Ran 最近被证明参与 LPS 内毒素信号转导。在这里，我们表明 I 型 c-Abl 与 Ran 相关。该复合物的形成是特定的，因为 Ran 不与高度同源的 IV 型 c-Abl 同种型结合。在未受刺激的淋巴 B 细胞中，I 型 c-Abl 酪氨酸激酶不活跃，而 IV 型激酶则活跃。 I 型 c-Abl/Ran 复合物的形成和 I 型 c-Abl 激酶活性的激活是 LPS 剂量依赖性的。该复合物在内毒素敏感的近交小鼠的 B 细胞中可检测到，但在内毒素抗性小鼠的 B 细胞中不存在。因此，这些发现表明 I 型 c-Abl 和 Ran 是脂多糖诱导的造血细胞生物反应的重要靶标。
Each of several isoforms of c-Abl may be involved in different biological functions. Type I c-Abl has been shown to be involved in LPS-induced differentiation and Type IV c-Abl, apoptosis. Ran has recently been shown to be involved in LPS endotoxin signal transduction. Here we show that Type I c-Abl associates with Ran. Formation of this complex is specific, as Ran did not associate with the highly homologous Type IV c-Abl isoform. In non-stimulated lymphoid B cells, Type I c-Abl tyrosine kinase is inactive, whereas Type IV kinase is active. Formation of Type I c-Abl/Ran complex and activation of Type I c-Abl kinase activity are LPS dose-dependent. This complex is detectable in B cells of endotoxin-sensitive inbred mice but absent in B cells of endotoxin-resistant mice. These findings therefore suggest that Type I c-Abl and Ran are important targets in lipopolysaccharide-induced biological responses of hematopoietic cells.