Preparation of truncated orf virus entry fusion complex proteins by chemical synthesis
Preparation of truncated orf virus entry fusion complex proteins by chemical synthesis
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DOI：
10.1002/psc.2627
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发表时间：
2014-06
影响因子：
2.1
通讯作者：
Ho-Lun Yeung;P. Harris;C. Squire;E. Baker;M. Brimble
中科院分区：
文献类型：
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作者：
Ho-Lun Yeung;P. Harris;C. Squire;E. Baker;M. Brimble
Chordopoxvirinae亚科的成员拥有一个不寻常的11蛋白入口融合复合体（EFC），该复合体高度保守，存在于所有物种中。这种EFC的作用方式是未知的，组成蛋白的相互作用也未被表征。本文介绍了ORFV036和049两种EFC蛋白的膜结构域截断线性结构体的化学合成。通过Boc固相肽合成和天然化学连接方法，这些截断的蛋白质很容易以毫克的量制备。这些强大的合成方案允许随时获得这些多肽，以促进生物学研究。版权所有©2014欧洲多肽协会和约翰威利父子有限公司
Members of the Chordopoxvirinae subfamily possess an unusual 11 protein entry–fusion complex (EFC) that is highly conserved and present in all species. The mode of action of this EFC is unknown, and the interactions of the constituent proteins are uncharacterised. Here, we present the chemical synthesis of membrane domain truncated linear constructs of two EFC proteins in orf virus, ORFV036 and 049. By using Boc solid phase peptide synthesis and native chemical ligation methods, these truncated proteins have been readily prepared in milligram quantities. These robust synthetic protocols allow ready access to these polypeptides to facilitate biological studies. Copyright © 2014 European Peptide Society and John Wiley & Sons, Ltd.