Synthesis of canavalin and concanavalin A in maturing Canavalia gladiata seeds
Synthesis of canavalin and concanavalin A in maturing Canavalia gladiata seeds
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成熟刀豆豆种子中刀豆球蛋白和刀豆球蛋白 A 的合成
DOI：
10.1093/oxfordjournals.pcp.a077312
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
T. Minamikawa
中科院分区：
文献类型：
--
作者：
D. Yamauchi;T. Minamikawa
在剑楠种子发育的不同阶段，采集剑楠种子，采用sds -凝胶电泳和蛋白印迹分析方法研究了剑楠种子中刀豆蛋白和刀豆蛋白A （Con A）积累的时间变化。结果表明，两种种子蛋白的合成早在开花后30天就开始了，且在种子发育过程中积累模式不同。菜豆蛋白的合成和积累在SOSO DAF时最为活跃，而Con A的含量则继续逐渐增加，直到种子成熟接近完成（80 DAF）。接下来，从40 DAF种子中制备RNA片段，在小麦胚芽系统中翻译，并通过免疫沉淀和sds凝胶电泳分析其翻译产物。用抗血清免疫沉淀的体外产物的摩尔重量（约48000）与canavalin非常接近。抗血清对Con A免疫沉淀的体外产物的mol wt（34,000）比Con A高约4,000，Canavalin-mRNA早在30 DAF时就存在，其翻译活性在35-40 DAF时最高。在30 DAF时也检测到Con A-mRNA，与canavalin-mRNA相比，Con A-mRNA的高活性保持到75 DAF。
In successive stages of seed development ofCanavalia gladiata, seeds were harvested, and time-course changes of the accumulation of canavalin and concanavalin A (Con A) were investigated by SDS-gel electrophoresis and protein blot analysis. Results showed that the synthesis of both seed proteins started as early as 30 days after flowering (DAF) and that they had different accumulation patterns during seed development. The synthesis and accumulation of canavalin were most active at SOSO DAF, whereas the content of Con A continued to increase gradually until the seed maturation was nearly completed (80 DAF). Next, an RNA fraction was prepared from seeds of 40 DAF, translated in a wheat germ system and its translation products analyzed by the immunoprecipitation and SDS-gel electrophoresis. The mol wt (about 48,000) of the in vitro product immunoprecipitated with the antiserum to canavalin was very closed to that of canavalin. The mol wt (34,000) of the in vitro product immunoprecipitated with the antiserum to Con A was about 4,000 higher than that of Con A. Canavalin-mRNA was present as early as at 30 DAF and its translational activity was the highest at 35–40 DAF. Con A-mRNA was also detected at 30 DAF and, in contrast to canavalin-mRNA, high activities were maintained until 75 DAF.