Adipose tissue extract promotes adipose tissue regeneration in an adipose tissue engineering chamber model
Adipose tissue extract promotes adipose tissue regeneration in an adipose tissue engineering chamber model
复制标题
脂肪组织提取物促进脂肪组织工程室模型中的脂肪组织再生
DOI：
10.1007/s00441-015-2322-5
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发表时间：
2016-05-01
影响因子：
3.6
通讯作者：
Lu, Feng
中科院分区：
文献类型：
--
作者：
Lu, Zijing;Yuan, Yi;Lu, Feng
建立了一种脂肪组织工程室模型，该模型能从现有的脂肪瓣中自发产生脂肪组织。然而，在该模型中，腔室没有完全填充脂肪组织。在这里，脂肪组织提取物（ATE）对脂肪组织再生的影响进行了研究。在体外，分别使用油红O和脂肪源性干细胞（ASCs）和大鼠主动脉内皮细胞（RAECs）上的管形成试验评价ATE的成脂和血管生成能力。在体内，植入1周后，将生理盐水或ATE注入脂肪组织工程室中。在注射后的不同时间点，对内容物进行形态计量学、组织学和免疫化学评价，并通过酶联免疫吸附测定（ELISA）和定量实时PCR分析生长因子和成脂基因的表达。除基线对照组（未将脂肪瓣插入腔室）外，所有组的脂肪瓣组织总体积均显著增加，尤其是ATE组。与对照组相比，ATE组的形态和结构更好，包膜更薄，血管更多。ATE组中血管生成生长因子和成脂标志物的表达显着较高。因此，ATE在脂肪组织工程室模型中显著促进脂肪组织再生并减少囊形成。这些数据表明，ATE通过释放也抑制囊形成的各种细胞因子和生长因子，为脂肪组织形成提供了更多的血管生成和脂肪生成微环境。
An adipose tissue engineering chamber model of spontaneous adipose tissue generation from an existing fat flap has been described. However, the chamber does not completely fill with adipose tissue in this model. Here, the effect of adipose tissue extract (ATE) on adipose tissue regeneration was investigated. In vitro, the adipogenic and angiogenic capacities of ATE were evaluated using Oil Red O and tube formation assays on adipose-derived stem cells (ASCs) and rat aortic endothelial cells (RAECs), respectively. In vivo, saline or ATE was injected into the adipose tissue engineering chamber 1 week after its implantation. At different time points post-injection, the contents were morphometrically, histologically, and immunohistochemically evaluated, and the expression of growth factors and adipogenic genes was analyzed by enzyme-linked immunosorbent assay (ELISA) and quantitative real-time PCR. With the exception of the baseline control group, in which fat flaps were not inserted into a chamber, the total volume of fat flap tissue increased significantly in all groups, especially in the ATE group. Better morphology and structure, a thinner capsule, and more vessels were observed in the ATE group than in the control group. Expression of angiogenic growth factors and adipogenic markers were significantly higher in the ATE group. ATE therefore significantly promoted adipose tissue regeneration and reduced capsule formation in an adipose tissue engineering chamber model. These data suggest that ATE provides a more angiogenic and adipogenic microenvironment for adipose tissue formation by releasing various cytokines and growth factors that also inhibit capsule formation.