An anther development F-box (ADF) protein regulated by tapetum degeneration retardation (TDR) controls rice anther development
An anther development F-box (ADF) protein regulated by tapetum degeneration retardation (TDR) controls rice anther development
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受绒毡层退化阻滞（TDR）调控的花药发育 F-box（ADF）蛋白控制水稻花药发育
DOI：
10.1007/s00425-014-2160-9
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发表时间：
2015-01-01
期刊：
影响因子：
4.3
通讯作者：
Yuan, Longping
中科院分区：
文献类型：
--
作者：
Li, Li;Li, Yixing;Yuan, Longping
水稻绒毡层是花药最内层的孢子体组织，在开花植物雄性生殖过程中起着重要的支持作用。F-box蛋白OsADF是TDR的直接靶蛋白之一，在水稻绒毡层细胞发育和花粉形成中起着重要作用。绒毡层细胞减数分裂后进行程序性细胞死亡（PCD），为花粉发育提供营养。以前我们发现绒毡层退化阻滞（TDR），一个基本的螺旋-环-螺旋转录因子，可以触发绒毡层PCD和控制花药发育过程中的花粉壁发育。然而，贸易和发展报告的全面监管网络仍有待调查。本研究克隆了水稻花药发育特异表达的F-box蛋白OsADF。通过qRT-PCR和RNA原位杂交进一步证实OsADF在花药发育的第9 - 12期绒毡层细胞中特异表达。与这种特异性表达模式相一致的是，OsADF的RNAi转基因株系表现出绒毡层退化和小孢子发育异常，最终产生育性降低的花粉。此外，我们还证明了水稻花药发育的关键调控因子TDR可以通过与OsADF启动子的E-box基序结合直接调控OsADF的表达。因此，本研究强调了TDR可能的调节作用，其通过触发可能的ADF介导的蛋白水解途径来调节绒毡层细胞发育和花粉形成。
In this study, we reported that a F-box protein, OsADF, as one of the direct targets of TDR , plays a critical role in rice tapetum cell development and pollen formation.The tapetum, the innermost sporophytic tissue of anther, plays an important supportive role in male reproduction in flowering plants. After meiosis, tapetal cells undergo programmed cell death (PCD) and provide nutrients for pollen development. Previously we showed that tapetum degeneration retardation (TDR), a basic helix-loop-helix transcription factor, can trigger tapetal PCD and control pollen wall development during anther development. However, the comprehensive regulatory network of TDR remains to be investigated. In this study, we cloned and characterized a panicle-specific expression F-box protein, anther development F-box (OsADF). By qRT-PCR and RNA in situ hybridization, we further confirmed that OsADF expressed specially in tapetal cells from stage 9 to stage 12 during anther development. In consistent with this specific expression pattern, the RNAi transgenic lines of OsADF exhibited abnormal tapetal degeneration and aborted microspores development, which eventually grew pollens with reduced fertility. Furthermore, we demonstrated that the TDR, a key regulator in controlling rice anther development, could regulate directly the expression of OsADF by binding to E-box motifs of its promoter. Therefore, this work highlighted the possible regulatory role of TDR, which regulates tapetal cell development and pollen formation via triggering the possible ADF-mediated proteolysis pathway.