DISCOVERY OF ENDOGENOUS CATECHOLAMINES IN LYMPHOCYTES AND EVIDENCE FOR CATECHOLAMINE REGULATION OF LYMPHOCYTE FUNCTION VIA AN AUTOCRINE LOOP
DISCOVERY OF ENDOGENOUS CATECHOLAMINES IN LYMPHOCYTES AND EVIDENCE FOR CATECHOLAMINE REGULATION OF LYMPHOCYTE FUNCTION VIA AN AUTOCRINE LOOP
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DOI：
10.1073/pnas.91.26.12912
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发表时间：
1994-12-20
影响因子：
11.1
通讯作者：
EWING, A
中科院分区：
文献类型：
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作者：
BERGQUIST, J;TARKOWSKI, A;EWING, A
已经获得的证据表明，儿茶酚胺及其代谢产物存在于单个淋巴细胞和提取物的T-和B-细胞克隆通过使用毛细管电泳电化学检测。酪氨酸羟化酶的药理学抑制降低了观察到的儿茶酚胺水平，表明淋巴细胞的儿茶酚胺合成。细胞内多巴胺水平被证明是由细胞外多巴胺增加，这表明细胞摄取机制。此外，与多巴胺或L-二羟基苯丙氨酸（多巴胺的前体）一起孵育，导致淋巴细胞增殖和分化的剂量依赖性抑制。总之，这些结果表明存在一个自分泌回路，淋巴细胞下调自己的活动。
Evidence has been obtained that catechol amines and their metabolites are present in single lymphocytes and extracts of T- and B-cell clones by use of capillary electrophoresis with electrochemical detection. Pharmacological inhibition of tyrosine hydroxylase reduces observed catecholamine levels, suggesting catecholamine synthesis by lymphocytes. Intracellular dopamine levels are shown to be increased by extracellular dopamine, suggesting a cellular-uptake mechanism. Furthermore, incubation with either dopamine or L-dihydroxyphenylalanine, a precursor of dopamine, results in a dose-dependent inhibition of lymphocyte proliferation and differentiation. Together, these results suggest the presence of an autocrine loop whereby lymphocytes down-regulate their own activity.