Local drug micro-injection to Ca enorhabdi ti s el eg ans with micro-gel beads
Local drug micro-injection to Ca enorhabdi ti s el eg ans with micro-gel beads
复制标题
微凝胶珠局部药物微注射给秀丽隐杆线虫
DOI：
10.1109/mhs.2013.6710408
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
T. Fukuda
中科院分区：
文献类型：
--
作者：
Naoya Nakanishi;M. Nakajima;N. Hisamoto;Masaru Takeuchi;M. Homma;T. Fukuda
基于光学显微镜(OM)和环境扫描电子显微镜(E-SEM)下的纳米操纵，提出了一种基于微吸管工具的荧光微凝胶微球局部药物注射方法。微凝胶微珠是由聚乙二醇二丙烯酸酯(PEGDA)制成的。这种药物在注射后会泄漏。微吸管工具的顶端通过注射器泵施加负压来附着微凝胶珠。秀丽隐杆线虫是疾病分析和神经分析的重要模式生物之一。为了进行体内实验，需要利用局部纳米注射技术将荧光材料或特定的生物有机体输送到特定的细胞内。该体系被认为是未来利用模式生物进行生命创新的纳米糖类体系的重要组成部分。
This paper presents a local drug micro-injection method by fluorescence micro-gel-bead with micro-pipette tool based on nano-manipulation under optical micro scope (OM) and environment scanning electron micro scope (E-SEM). Micro-gel-bead is fabricated from Polyethylene Glycol Diacrylate (PEG-DA). The drug is leaked after injection. The tip of micro-pipette tool is used to attach micro-gel-beads by applying a negative pressure with syringe pump. The Caenorhabditi selegans (C.elegans) is used as a target biological specimen, which is one of the important model organism for disease analysis and nerve analysis. The local-nano-injection technique is needed to transport fluorescent materials or specific biological organism into specific cell for in-vivo experiment. The proposed system is considered to be important as future nano-sugery system for life innovation using model organism.