Analysis of the early immune response to infection by infectious bursal disease virus in chickens differing in their resistance to the disease.
Analysis of the early immune response to infection by infectious bursal disease virus in chickens differing in their resistance to the disease.
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DOI：
10.1128/jvi.02828-14
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发表时间：
2015-03
影响因子：
5.4
通讯作者：
Burt DW
中科院分区：
文献类型：
--
作者：
Smith J;Sadeyen JR;Butter C;Kaiser P;Burt DW
利用鸡全基因组基因表达谱芯片研究了传染性法氏囊病病毒（IBDV）感染后宿主的反应。脾和法氏囊组织进行了检查，从控制和感染的鸡在感染后2，3，和4天，从两个线，不同的抗IBDV感染。在此期间的主机响应进行了评估，并检查易感和耐药鸡品系之间的差异。抗病毒基因，包括IFNA、IFNG、MX1、IFITM1、IFITM3和IFITM5，在感染后上调。对这些基因表达数据的评估使我们能够预测几个基因作为参与IBDV抗性的候选基因。重要性传染性法氏囊病（IBD）对家禽业具有重要的经济意义，因此对粮食安全也很重要。疫苗是可用的，但病毒的野外毒株的毒性越来越强。因此，迫切需要探索新的控制解决方案，其中之一是培育对IBD具有更大抵抗力的鸟类。在所有农场动物物种中，家禽可能是唯一可以实现这一目标的，因为全球每年生产的600亿只鸡中有85%的基因基本上由两家育种公司控制。在一项全面的研究中，我们试图在这里确定全球转录组的差异，在不同的抗性鸡株之间的病毒的靶器官，并预测候选抗性基因。
Chicken whole-genome gene expression arrays were used to analyze the host response to infection by infectious bursal disease virus (IBDV). Spleen and bursal tissue were examined from control and infected birds at 2, 3, and 4 days postinfection from two lines that differ in their resistance to IBDV infection. The host response was evaluated over this period, and differences between susceptible and resistant chicken lines were examined. Antiviral genes, including IFNA, IFNG, MX1, IFITM1, IFITM3, and IFITM5, were upregulated in response to infection. Evaluation of this gene expression data allowed us to predict several genes as candidates for involvement in resistance to IBDV. IMPORTANCE Infectious bursal disease (IBD) is of economic importance to the poultry industry and thus is also important for food security. Vaccines are available, but field strains of the virus are of increasing virulence. There is thus an urgent need to explore new control solutions, one of which would be to breed birds with greater resistance to IBD. This goal is perhaps uniquely achievable with poultry, of all farm animal species, since the genetics of 85% of the 60 billion chickens produced worldwide each year is under the control of essentially two breeding companies. In a comprehensive study, we attempt here to identify global transcriptomic differences in the target organ of the virus between chicken lines that differ in resistance and to predict candidate resistance genes.