Amoxicillin and specific bacteriophage can be used together for eradication of biofilm of Klebsiella pneumoniae B5055
Amoxicillin and specific bacteriophage can be used together for eradication of biofilm of Klebsiella pneumoniae B5055
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DOI：
10.1007/s11274-009-9991-8
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发表时间：
2009-07-01
影响因子：
4.1
通讯作者：
Chhibber, Sanjay
中科院分区：
文献类型：
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作者：
Bedi, Manmeet Sakshi;Verma, Vivek;Chhibber, Sanjay
尽管抗生素和噬菌体在治疗细菌感染方面有疗效，但它们在治疗生物被膜相关感染中的作用仍在考虑中，特别是在较老的生物被膜的情况下。在这里，我们评价了单用噬菌体或与阿莫西林合用来根除肺炎克雷伯菌B5055生物被膜的效果。将96孔板上培养的肺炎克雷伯菌B5055的浮游细胞和生物膜分别以不同的感染复数(MOI)和3种不同的抗生素浓度(512、256和128µg/ml)暴露于噬菌体和阿莫西林。256mU g/ml阿莫西林处理后，浮游生物膜和1日龄生物膜的细菌负荷分别下降了对数倍4.1/-A0.31(P=0.008)和1.2 4+/-A0.2 7(P=0.0 5)，而成熟生物膜(8日龄)的细菌负荷下降不明显(P=0.2 3)。8日龄的生物膜暴露于较高的抗生素浓度(512mU g/ml)和单独的噬菌体(MOI=0.01)时，对数值分别下降2.97+/-A0.11(P=0.182)和3.51+/-A0.19(P=0.073)。而同时暴露于阿莫西林和噬菌体时，生物被膜的细菌负荷显著降低(P<；0.01)。因此，当抗生素与特定的噬菌体联合使用时，对生物膜结构的破坏更大，这表明噬菌体可以成功地与抗生素治疗一起使用。联合疗法的另一个优势是它能够阻止耐药突变的形成，否则单用噬菌体或抗生素就很容易产生耐药突变。
Despite the efficacy of antibiotics as well as bacteriophages in treatment of bacterial infections, their role in treatment of biofilm associated infections is still under consideration especially in case of older biofilms. Here, efficacy of bacteriophage alone or in combination with amoxicillin, for eradication of biofilm of Klebsiella pneumoniae B5055 has been assessed. Planktonic cells as well as biofilm of K. pneumoniae B5055 grown in 96-well microtiter plates were exposed to bacteriophage and amoxicillin at various Multiplicity of Infections (MoIs) as well as at three different antibiotic concentrations (512, 256 and 128 mu g/ml), respectively. After exposure to 256 mu g/ml (MIC) of amoxicillin, bacterial load of planktonic culture as well as 1-day-old biofilm was reduced by a log factor of 4.1 +/- A 0.31 (P = 0.008) and 1.24 +/- A 0.27 (P < 0.05), respectively but reduction in the bacterial load of mature biofilm (8-day-old) was insignificant (P = 0.23). When 8-day-old biofilm was exposed to higher antibiotic concentration (512 mu g/ml) or phage alone (MoI = 0.01) a log reduction of 2.97 +/- A 0.11 (P = 0.182) and 3.51 +/- A 0.19 (P = 0.073), respectively was observed. While on exposing to a combination of both the amoxicillin and phage, a significant reduction (P < 0.01) in bacterial load of the biofilm was seen. Hence, when antibiotic was used in combination with specific bacteriophage a greater destruction of the biofilm structure suggested that the phages could be used successfully along with antibiotic therapy. An added advantage of the combination therapy would be its ability to check formation of resistant mutants that otherwise develop easily upon using phage or antibiotic alone.