Whole genome analysis of the marine Bacteroidetes 'Gramella forsetii' reveals adaptations to degradation of polymeric organic matter
Whole genome analysis of the marine Bacteroidetes 'Gramella forsetii' reveals adaptations to degradation of polymeric organic matter
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DOI：
10.1111/j.1462-2920.2006.01152.x
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发表时间：
2006-12-01
影响因子：
5.1
通讯作者：
Gloeckner, Frank Oliver
中科院分区：
文献类型：
--
作者：
Bauer, Margarete;Kube, Michael;Gloeckner, Frank Oliver
拟杆菌门的成员以前称为噬细胞-黄细菌-拟杆菌门(CFB)，是经常在宏观有机物颗粒（海洋雪）上发现的海洋异养浮游细菌的主要分类群之一。此外，它们还被证明代表了营养丰富的微环境中自由生活的微生物群落的重要组成部分。它们的丰度和分布模式与酶活性研究相结合，得出这样的观点：该组生物是降解海洋有机物池中溶解部分和颗粒部分中高分子量化合物的专家，这意味着拟杆菌在海洋碳循环中发挥着重要作用。尽管它们具有重要的生态意义，但迄今为止，有关该类生物体的全面分子数据仍然很少。在这里，我们报告了海洋拟杆菌代表“Gramella forsetii”KT0803 的首次全基因组分析。预测的蛋白质组的功能分析揭示了几个特征，综合考虑，这些特征表明这种海洋拟杆菌对高分子量有机物的降解具有明显的适应性，例如编码水解酶的大量基因、预测的对聚合碳源的偏好以及独特的表面粘附能力。
Members of the Bacteroidetes, formerly known as the Cytophaga-Flavobacteria-Bacteroides (CFB) phylum, are among the major taxa of marine heterotrophic bacterioplankton frequently found on macroscopic organic matter particles (marine snow). In addition, they have been shown to also represent a significant part of free-living microbial assemblages in nutrient-rich microenvironments. Their abundance and distribution pattern in combination with enzymatic activity studies has led to the notion that organisms of this group are specialists for degradation of high molecular weight compounds in both the dissolved and particulate fraction of the marine organic matter pool, implying a major role of Bacteroidetes in the marine carbon cycle. Despite their ecological importance, comprehensive molecular data on organisms of this group have been scarce so far. Here we report on the first whole genome analysis of a marine Bacteroidetes representative, 'Gramella forsetii' KT0803. Functional analysis of the predicted proteome disclosed several traits which in joint consideration suggest a clear adaptation of this marine Bacteroidetes representative to the degradation of high molecular weight organic matter, such as a substantial suite of genes encoding hydrolytic enzymes, a predicted preference for polymeric carbon sources and a distinct capability for surface adhesion.