Purification of Interferon γ-Interferon γ Receptor Complexes by Preparative Electrophoresis on Native Gels
Purification of Interferon γ-Interferon γ Receptor Complexes by Preparative Electrophoresis on Native Gels
复制标题
通过非变性凝胶制备型电泳纯化干扰素 γ-干扰素 γ 受体复合物
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
M. Manneberg
中科院分区：
文献类型：
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作者：
M. Fountoulakis;E. Takacsdilorenzo;J. Juranville;M. Manneberg
摘要 某些研究需要细胞因子-受体复合物，包括结晶、核磁共振谱和细胞因子生物反应机制的研究。配体-受体复合物的纯度对于大多数此类应用至关重要。我们研究了通过非变性凝胶电泳纯化蛋白质-蛋白质复合物的可能性。从部分纯化的小鼠蛋白和高度纯化的人蛋白开始，我们通过在非变性聚丙烯酰胺凝胶上进行制备型电泳制备了毫克量的干扰素γ-干扰素γ受体复合物。在这两种情况下，都回收了具有正确结合化学计量的纯配体-受体复合物。制备型天然凝胶上的电泳可能被证明对于制备用于各种研究的蛋白质-蛋白质复合物具有普遍意义。
Abstract Cytokine-receptor complexes are required for certain studies including crystallization, NMR spectra, and investigation of the biological response mechanism to the cytokine. The purity of the ligand-receptor complex is critical for most of these applications. We investigated the possibility of purifying protein-protein complexes by electrophoresis on native gels. Starting with partially purified mouse and highly purified human proteins, we prepared milligram amounts of interferon γ-interferon γ receptor complexes by preparative electrophoresis on nondenaturing polyacrylamide gels. In both cases, pure ligand-receptor complexes with the correct stoichiometry of binding were recovered. Electrophoresis on preparative native gels may prove to be of general interest for the preparation of protein-protein complexes to be used in diverse studies.