Comprehensive genomic identification and expression analysis of the nucleobase-ascorbate transporter (NAT) gene family in apple
Comprehensive genomic identification and expression analysis of the nucleobase-ascorbate transporter (NAT) gene family in apple
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DOI：
10.1016/j.scienta.2015.09.034
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发表时间：
2016-01
影响因子：
4.3
通讯作者：
Tingting Sun;Dongfeng Jia;Linlin Huang;Yun Shao;F. Ma
中科院分区：
文献类型：
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作者：
Tingting Sun;Dongfeng Jia;Linlin Huang;Yun Shao;F. Ma
核碱基抗坏血酸转运蛋白（NATs），又称核碱基阳离子同向转运蛋白2 （NCS2）蛋白，是一种在进化上广泛存在的转运蛋白家族。由于对苹果（Malus domestica）的natas基因知之甚少，本文通过筛选苹果（Malus domestica） GDR基因组数据库，鉴定出该物种的14个natas基因。本文综述了该基因家族在苹果中的结构、染色体分布和定位、系统发育和基序。系统发育和结构分析表明，这些基因可划分为3个簇。这里选择的14个基因在不同组织中表现出不同的表达模式。在茎尖、成熟叶、幼果和成熟果中，mdnat12、MdNAT4和mdnat6的表达量非常高。随着果实的成熟，mdnat1和mdnat7的表达有增加的趋势。mdnat6对干旱和盐度均有响应。在果实成熟过程中，以及在干旱或盐胁迫下，它们的转录水平普遍显著上调。结果表明，该家族成员在组织发育，果实成熟和植物适应不利生长环境中起作用。
Nucleobase ascorbate transporters (NATs), also known as nucleobase:cation-symporter 2 (NCS2) proteins, belong to an evolutionarily widespread family of transport proteins. Because little is known aboutNATgenes in apple (Malus domestica), this papper screened GDR genome databases and identified 14NATs in that species. Here, presented an overview of this gene family in apple, including structures, chromosome distribution and localization, phylogenies, and motifs. Phylogenetic and structure analyses showed that these genes could be assigned to three clusters. The 14 genes selected here displayed differential expression patterns in various tissues. Expression was very high forMdNAT12,MdNAT4, andMdNAT6in the shoot tips, mature leaves, and young and mature fruits.MdNAT1andMdNAT7tended to show increased expression while the fruits were maturing.MdNAT6proved responsive to both drought and salinity. Their transcript levels were generally up-regulated significantly during the fruit maturation process as well as in response to the imposition of drought or salt stress. Results suggest that members of this family function in tissue development, fruit maturation, and plant adaptations to adverse growing environments.