Generation of natural killer cells from serum-free, expanded human umbilical cord blood CD34+ cells
Generation of natural killer cells from serum-free, expanded human umbilical cord blood CD34+ cells
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DOI：
10.1089/scd.2007.0033
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发表时间：
2007-12-01
影响因子：
4
通讯作者：
Hwang, Shiaw-Min
中科院分区：
文献类型：
--
作者：
Kao, I-Ting;Yao, Chao-Ling;Hwang, Shiaw-Min
自然杀伤（NK）细胞是先天免疫系统的重要效应细胞，其表现出针对感染因子和肿瘤细胞的细胞溶解活性。NK细胞来源于CD 34（+）造血干细胞（HSC）。人脐带血是一种丰富的造血干细胞来源。在此之前，我们已经报道了一种优化的无血清培养基，用于从UCB中体外扩增CD 34（+）细胞。本研究检测了无血清扩增的CD 34（+）细胞向NK细胞的分化及其诱导动力学。诱导5周后，通过分析表面抗原、IFN-γ分泌和对K562细胞的细胞毒性来表征诱导的NK细胞。结果表明，无血清扩增的CD 34（+）细胞来源的NK细胞具有NK细胞的特性和功能。此外，无血清扩增的CD 34（+）细胞显示出比新鲜分离的CD 34（+）细胞显著更高的NK细胞分化潜能。与新鲜分离的CD 34（+）细胞相比，无血清扩增的CD 34（+）细胞诱导的NK细胞显示出更高的IFN-γ分泌浓度和细胞毒性能力。因此，体外扩增的CD 34（+）细胞在优化的无血清培养基中可以分化为NK细胞，并为免疫治疗方法提供了有希望的细胞来源。
Natural killer (NK) cells are important effectors of the innate immune system, which exhibits cytolytic activity against infectious agents and tumor cells. NK cells are derived from CD34(+) hematopoietic stem cells (HSCs). Human umbilical cord blood (UCB) has been recognized as a rich source of HSCs. Previously, we have reported an optimized serum-free medium for ex vivo expansion of CD34(+) cells from UCB. In this study, the serum-free, expanded CD34(+) cells were tested to differentiate into NK cells and their induction kinetics. After 5 weeks of induction, the induced NK cells were characterized by analysis of surface antigens, IFN-gamma secretion, and cytotoxicity against K562 cells. The results indicated that NK cells derived from the serum-free, expanded CD34(+) cells exhibited both characteristics and functions of NK cells. Furthermore, the serum-free, expanded CD34(+) cells showed a significantly higher NK cell differentiation potential than freshly isolated CD34(+) cells. NK cells induced from serum-free, expanded CD34(+) cells showed a higher concentration of IFN-gamma secretion and ability of cytotoxicity than those from freshly isolated CD34(+) cells. Therefore, ex vivo-expanded CD34(+) cells in optimized serum-free medium could differentiate into NK cells and provided a promising cell source for immunotherapeutic approaches.