A comparison of video and magnetic search coil recordings of mouse eye movements
A comparison of video and magnetic search coil recordings of mouse eye movements
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DOI：
10.1016/s0165-0270(00)00218-1
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发表时间：
2000-06-30
影响因子：
3
通讯作者：
De Zeeuw, CI
中科院分区：
文献类型：
--
作者：
Stahl, JS;van Alphen, AM;De Zeeuw, CI
对分子生物学和行为学的联系的兴趣激发了对小鼠眼球运动的研究。不幸的是，记录这种小动物的眼球运动在技术上是困难的。我们提出了第一种方法，获得校准的视频眼电图，并与同时获得的巩膜搜索线圈记录在C57 BL/6色素小鼠的结果进行对比。我们确定的瞳孔从角膜曲率中心的距离，在相机运动过程中的瞳孔和角膜反射的相对运动的基础上，并使用该距离转换瞳孔运动的后续视频测量到眼睛旋转。我们在光线中记录了正弦旋转（0.1-1.6 Hz）期间的反应，在搜索线圈植入前通过视频记录，以及在植入后同时通过视频和搜索线圈记录。植入前，视频衍生增益范围为0.1 Hz时的0.86 +/- 0.03（平均值+/- SD）至0.8 Hz时的0.95 +/- 0.03。相位从0.1 Hz时的-3.1 +/- 2.6度（眼导联头部）单调进展到1.6 Hz时的+ 5.9 +/- 1.1度。弹簧圈植入将视频衍生增益的范围降低至0.64-0.79。这种减少反映了线圈对正常行为的破坏。线圈数据证实了视频结果。视频和搜索线圈技术各有优势。在设计和解释使用线圈技术的实验时，需要采取特定的预防措施。(C)2000 Elsevier Science B. V.保留所有权利。
Interest in connecting molecular biology and behavior is motivating research into the eye movements of mice. Unfortunately, recording eye movements in this diminutive animal is technically difficult. We present the first method for obtaining calibrated video oculography, and contrast the results with simultaneously obtained scleral search coil recordings in C57BL/6 pigmented mice. We determined the distance of the pupil from the center of corneal curvature, based upon relative motions of the pupil and corneal reflections during camera movements, and used the distance to convert subsequent video measurements of pupil motion to eye rotation. We recorded responses during sinusoidal rotation (0.1-1.6 Hz) in the light, by video prior to search coil implantation, and by video and search coil simultaneously following implantation. Pre-implantation, video-derived gains ranged from 0.86 +/- 0.03 (mean +/- SD) at 0.1 Hz to 0.95 +/- 0.03 at 0.8 Hz. Phase progressed monotonically from - 3.1 +/- 2.6 degrees (eye leads head) at 0.1 Hz to + 5.9 +/- 1.1 degrees at 1,6 Hz. Coil implantation reduced the range of video-derived gains to 0.64-0.79. This reduction reflects disruption of normal behavior by the coil. Coil data confirmed the video results. Video and search coil techniques each have advantages. Specific precautions are required when designing and interpreting experiments using the coil technique. (C) 2000 Elsevier Science B.V. All rights reserved.