Protein-RNA contacts in Sendai virus nucleocapsids revealed by photo-crosslinking.
Protein-RNA contacts in Sendai virus nucleocapsids revealed by photo-crosslinking.
复制标题
通过光交联揭示仙台病毒核衣壳中的蛋白质-RNA 接触。
DOI：
10.1016/0042-6822(79)90546-4
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发表时间：
1979
期刊：
影响因子：
3.7
通讯作者：
D. Kingsbury
中科院分区：
文献类型：
--
作者：
R. Raghow;D. Kingsbury
仙台病毒核衣壳的基因组RNA中的蛋白质种类和核酸碱基之间的接触进行了检查紫外线（uv）诱导的交联。核衣壳结构单位NP和参与病毒转录的丰度较低的蛋白质P都有效地与RNA连接，表明这两种蛋白质都与RNA碱基接触。然而，RNA作为完整核衣壳中病毒RNA聚合酶的模板，因此这些接触必须是可逆的或仅限于碱基的非编码区。
Contacts between protein species and nucleic acid bases in the genomic RNA of Sendai virus nucleocapsids were examined by ultraviolet light (uv)-induced crosslinking. Both NP, the nucleocapsid structure unit, and P, the less abundant protein species implicated in viral transcription, were efficiently linked to the RNA, indicating that both species contact RNA bases. However, the RNA serves as template for viral RNA polymerases in the intact nucleocapsid, so these contacts must either be reversible or restricted to noncoding regions of the bases.