Quantitative in situ correlation between microscopic MRI and polarized light microscopy studies of articular cartilage
Quantitative in situ correlation between microscopic MRI and polarized light microscopy studies of articular cartilage
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DOI：
10.1053/joca.2000.0405
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发表时间：
2001-07-01
影响因子：
7
通讯作者：
Lust, G
中科院分区：
文献类型：
--
作者：
Xia, Y;Moody, JB;Lust, G
目的：在尽可能高的MRI分辨率下，建立磁共振显微镜（mu MRI）无创成像与偏振光显微镜（PLM）组织学成像之间的相关性（13.7 μ m），并且基于相同的组织片（来自犬肩关节的关节软骨）。在μ MRI实验中，分析了质子强度图像的层状外观（魔角效应）和T（2）弛豫图像的各向异性特征。在PLM实验中，我们构建了软骨中光延迟和胶原纤维取向的二维图像。结果：T（2）曲线具有明显的不对称钟形曲线和三个特征区。延迟曲线在组织的过渡区的中间具有非零最小值。角度轮廓在过渡区上具有平滑变化。这些事实表明，在过渡区的胶原纤维不是完全随机的，但有一个剩余的顺序。此外，T（2）曲线的峰值与延迟曲线的最小值重合，两者都代表组织的最各向同性区域。发现双曲正切函数最能描述软骨中胶原纤维的过渡。一组标准的开发，为每种技术来定义的功能，在定量measurement.Conclusions：该标准提供了，第一次，一组定量和客观的手段来细分组织厚度的区域，在组织学和MRI。结果表明，基于T（2）特征的mu MRI区域在统计学上等同于基于胶原纤维方向的组织学区域（浅表、过渡、放射状区域和总厚度的t概率分别为0.730、0.973、0.647、0.850）。(C)2001年国际骨关节炎研究学会。
Objective: To establish the correlation between the non-invasive imaging by magnetic resonance microscopy (mu MRI) and the histological imaging by polarized light microscopy (PLM) accurately, quantitatively, at the highest possible MRI resolution (13.7 mum), and based on the same piece of tissue (articular cartilage from canine shoulder joint).Design: In mu MRI experiments, the laminar appearance (the magic angle effect) of the proton intensity images and the anisotropic characteristics of the T(2) relaxation images were analysed. In PLM experiments, the images of the optical retardation and collagen-fibre orientation in cartilage were constructed in two dimensions,Results: The T(2) profile has a distinctly asymmetric bell-shaped curve and three featured zones. The retardation profile has a non-zero minimum at the middle of the transitional zone of the tissue. The angle profile has a smooth variation across the transitional zone. These facts suggest that the collagen fibres in the transitional zone are not entirely random but have a residual order. In addition, the peak of the T(2) Profile coincides with the minimum of the retardation profile, both represent the most isotropic region of the tissue. A hyperbolic tangent function was found to best describe the transition of the collagen fibres in cartilage. A set of criteria was developed for each technique to define the features in the quantitative measurements.Conclusions: The criteria offer, for the first time, a set of quantitative and objective means to subdivide the tissue thickness into the zones in histology and in MRI. It is shown that the mu MRI zones based on the T(2) characteristics are statistically equivalent to the histological zones based on the collagen fibre orientation (t-probabilities of 0.730, 0.973, 0.647, 0.850 for the superficial, transitional, radial zones and the total thickness). (C) 2001 OsteoArthritis Research Society International.