Some effects of glucose and calcium upon the metabolism of kidney slices from adult and newborn rats.
Some effects of glucose and calcium upon the metabolism of kidney slices from adult and newborn rats.
复制标题
葡萄糖和钙对成年和新生大鼠肾切片代谢的一些影响。
DOI：
10.1042/bj0450068
复制
发表时间：
1949
期刊：
影响因子：
--
通讯作者：
J. Robinson
中科院分区：
文献类型：
--
作者：
J. Robinson
Cutting & McCance B）比较了从新生和成年动物的肾脏切下的切片的Meta。他们发现，新生大鼠肾脏组织的呼吸速率在2小时内几乎没有变化，而成人组织的细胞迅速脱落。这种下降经常被报道，激光认为这是肾脏阿萨特点。然而，Cutting & McCance指出，他们的成年组织切片在2小时后明显崩解，并且经常损失40- 50%的氮。将这些切片浸入pH 7-4的磷酸盐缓冲溶液中，类似于Krebs所描述的溶液。它不含钙，磷酸盐浓度约为哺乳动物细胞外液中的8倍。切割还研究了影响的钙后的呼吸和氮的损失的肾片从成人和新生儿钙不会留在含有磷酸盐的溶液中，在pH值7 - 4，他们的比较在缓冲的生理盐水溶液中与磷酸盐在pH值6-8和碳酸氢钠-林格在钙，率保持不变，但仍然远远不是恒定的。
Cutting & McCance b) compared the meta- bolism of slices cut from the kidneys of newborn and adult animals. They found that the respiratory rate of kidney tissue from newborn rats changed little over a period of 2 hr., whilst that of the adult tissue fell off rapidly. Such a decline has often been reported, and Laser acceptedit asa peculiarity of the kidney. Cutting & McCance noted, however, that their slices of adult tissue were visibly dis- integrating after 2 hr., and had often lost 40-50 % of their nitrogen. These slices were immersed in a solution buffered with phosphate at pH 7-4, similar to that described by Krebs It contained no calcium, andhad about eight times the concentration of phosphate found in mammalian extracellular fluids. Cutting also studied the effect of calcium upon the respiration and the loss of nitrogen of kidney slices from adult and newborn calcium would not remain in a solution containing phosphate at pH 7 4, their comparisons in a saline solution buffered with phosphate at pH 6-8 and in bicar-bonate-Ringer The in the calcium, rate was maintained, but was still far from constant.