Transcriptional regulation of human 3'-phosphoadenosine 5'-phosphosulfate synthase 1.
Transcriptional regulation of human 3'-phosphoadenosine 5'-phosphosulfate synthase 1.
复制标题
人 3-磷酸腺苷 5-磷酸硫酸合酶 1 的转录调控。
DOI：
10.1006/bbrc.2001.5032
复制
发表时间：
2001
影响因子：
3.1
通讯作者：
Strott,CA
中科院分区：
文献类型：
--
作者：
Shimizu,C;Fuda,H;Lee,YC;Strott,CA
磺化对于内部环境的正常生长、发育和维持至关重要，需要通用磺酸盐供体分子 3'-磷酸腺苷 5'-磷酸硫酸盐 (PAPS)，由 ATP 和无机硫酸盐通过两种双功能 PAPS 合酶同工酶产生。发现既不包含 TATA 也不包含 CCAAT 盒的 PAPS 合酶 1 基因受到转录因子 Sp1 家族的影响。近端启动子区域存在多个 GC/GT 盒，缺失分析表明它们参与转录，这一发现得到了特定 GC/GT 盒突变分析的支持。 SW13 细胞的核提取物高度表达 PAPS 合酶 1，含有与具有特定 GC/GT 盒的探针结合的蛋白质；此外，通过超位移分析证实了核提取物中 Sp1、Sp2 和 Sp3 蛋白的存在。使用 SL2 细胞的共转染实验为 Sp1 参与 PAPS 合酶 1 基因的转录调控提供了额外的支持。 Sp2 和/或 Sp3 的参与目前尚不清楚。
Sulfonation, which is essential for normal growth, development and maintenance of the internal milieu, requires the universal sulfonate donor molecule 3′-phosphoadenosine 5′-phosphosulfate (PAPS) produced from ATP and inorganic sulfate by two bifunctional PAPS synthase isozymes. The gene for PAPS synthase 1 containing neither a TATA nor a CCAAT box was found to be under the influence of the Sp1 family of transcription factors. Multiple GC/GT boxes are present in the proximal promoter region and deletion analysis implicated their involvement in transcription, a finding supported by mutational analysis of specific GC/GT boxes. Nuclear extract of SW13 cells, which highly express PAPS synthase 1, contains proteins that bind to probes possessing specific GC/GT boxes; furthermore, the presence of Sp1, Sp2, and Sp3 proteins in nuclear extracts was confirmed by supershift analysis. Cotransfection experiments using SL2 cells yielded additional support for the involvement of Sp1 in transcriptional regulation of the PAPS synthase 1 gene; the involvement of Sp2 and/or Sp3 is presently unclear.