Different assemblies of the DAM1 complex follow shortening microtubules by distinct mechanisms
Different assemblies of the DAM1 complex follow shortening microtubules by distinct mechanisms
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DOI：
10.1073/pnas.0801811105
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发表时间：
2008-05-13
影响因子：
11.1
通讯作者：
Mclntosh, J. R.
中科院分区：
文献类型：
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作者：
Grishchuk, E. L.;Spiridonov, I. S.;Mclntosh, J. R.
有丝分裂染色体在缩短的纺锤体微管 (MT) 末端分离。在芽殖酵母中，Dam1 多蛋白复合物支持这种动态附着，从而有助于准确的染色体分离。 Purified Dam1 将追踪解聚 MT 的末端，并可将其与体外微珠运输偶联。人们认为这种运动的持续性取决于 Dam1 复合体可以在 MT 周围形成的环，但尚未考虑替代耦合几何形状对这些运动做出贡献的可能性。在这里，我们证明环和非环 Dam1 低聚物都可以跟踪 MT 末端并实现体外货物的持续运动。然而，这两个组件的耦合特性非常不同，因此每个组件都可能对染色体运动做出不同的贡献。
Mitotic chromosomes segregate at the ends of shortening spindle microtubules (MTs). in budding yeast, the Dam1 multiprotein complex supports this dynamic attachment, thereby contributing to accurate chromosome segregation. Purified Dam1 will track the end of a depolymerizing MT and can couple it to microbead transport in vitro. The processivity of such motions has been thought to depend on rings that the Dam1 complex can form around MTs, but the possibility that alternative coupling geometries contribute to these motilities has not been considered. Here, we demonstrate that both rings and nonencircling Dam1 oligomers can track MT ends and enable processive cargo movement in vitro. The coupling properties of these two assemblies are, however, quite different, so each may make a distinct contribution to chromosome motility.