Characterization of two related Epstein-Barr virus-encoded membrane proteins that are differentially expressed in Burkitt lymphoma and in vitro-transformed cell lines.
Characterization of two related Epstein-Barr virus-encoded membrane proteins that are differentially expressed in Burkitt lymphoma and in vitro-transformed cell lines.
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两种相关的 Epstein-Barr 病毒编码膜蛋白的表征，这两种蛋白在伯基特淋巴瘤和体外转化细胞系中差异表达。
DOI：
10.1073/pnas.83.15.5703
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发表时间：
1986
影响因子：
11.1
通讯作者：
H. Wolf
中科院分区：
文献类型：
--
作者：
S. Modrow;H. Wolf
Epstein-Barr 病毒的两个相关但差异表达的潜在膜蛋白由病毒基因组 EcoRI D het 区域中的相同阅读框编码。通过计算机辅助预测二级结构来选择这些蛋白质氨基酸序列中的潜在抗原位点，并化学合成与位于蛋白质不同部分的区域相对应的两个寡肽。这些肽的兔抗血清用于对来自不同来源的 Epstein-Barr 病毒阳性细胞系的天然病毒蛋白进行免疫沉淀。两种预测的膜蛋白都可以从体外用 EBV 转化的细胞系或传染性单核细胞增多症患者的细胞系中沉淀出来。在伯基特淋巴瘤建立的细胞系中，只能鉴定出较小的多肽，其氨基末端缺少 138 个氨基酸。在 ELISA 中使用合成肽作为抗原，我们检测到传染性单核细胞增多症和鼻咽癌患者血清中抗体滴度升高。
Two related but differentially expressed potential membrane proteins of Epstein-Barr virus are encoded by the same reading frame in the EcoRI D het region of the viral genome. Potential antigenic sites in the amino acid sequence of these proteins were selected by computer-aided prediction of the secondary structure and two oligopeptides corresponding to regions located in different parts of the proteins were synthesized chemically. Rabbit antisera to these peptides were used for immunoprecipitation of the native viral proteins from Epstein-Barr virus-positive cell lines from various sources. Both predicted membrane proteins could be precipitated from cell lines that had been transformed in vitro with EBV or from cell lines derived from infectious mononucleosis patients. In cell lines established from Burkitt lymphoma, only the smaller polypeptide, which lacks 138 amino acids from the amino terminus, could be identified. Using the synthetic peptides as antigens in ELISA, we detected elevated antibody titers in sera from patients with infectious mononucleosis and nasopharyngeal carcinoma.