Cell-mediated immune responses to human papillomavirus 16 E6 and E7 antigens as measured by interferon gamma enzyme-linked immunospot in women with cleared or persistent human papillomavirus infection.
Cell-mediated immune responses to human papillomavirus 16 E6 and E7 antigens as measured by interferon gamma enzyme-linked immunospot in women with cleared or persistent human papillomavirus infection.
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DOI：
10.1111/igc.0b013e3181a388c4
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发表时间：
2009-05
期刊：
影响因子：
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通讯作者：
Moscicki AB
中科院分区：
文献类型：
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作者：
Farhat S;Nakagawa M;Moscicki AB
细胞介导的免疫反应在控制HPV感染中被认为是重要的。我们使用干扰素-γ酶联免疫斑点试验(ELISpot)检测了感染HPV16型的妇女外周血中对HPV16E6和E7的细胞免疫反应，并将这些妇女与HPV16型持续感染的妇女进行了比较。一旦通过聚合酶链式反应检测到宫颈HPV16感染，就招募了参与宫颈HPV纵向研究的妇女。对四组妇女进行了检查：1)持续性，2)间歇性，3)暂时性，4)痊愈。对55名女性的96份样本进行了比较。比较干扰素-γELISpot和HPV16清除，最近持续的10名妇女中，没有一人对E6或E7有反应；14/24名最近清除的妇女有E6反应，8/24人有E7反应。间歇性坚持的妇女与最近坚持的妇女的表现类似：50%的妇女E6阳性，20%的妇女E7阳性。总而言之，抗E6反应似乎对立即控制HPV至关重要，在一些女性中，如果HPV感染后不立即消除，最终会产生免疫耐受。
Cell mediated immune responses have been thought to be important in the control of HPV infections. We examined cell-mediated immune responses to HPV 16 E6 and E7 in the peripheral blood using IFN-γ enzyme-linked ImmunoSpot (ELISpot) assay in women with HPV 16 infection who showed clearance and compared these women to women with HPV 16 persistence. Women participating in a longitudinal study of cervical HPV were recruited once cervical HPV 16 infection was detected by PCR. Four groups of women were examined; 1) persistent 2) intermittent 3) transient and 4) cleared. Ninety-six samples from 55 women were compared. Comparing IFN-γ ELISpot to HPV 16 clearance, of 10 women with recent persistence, none had response to either E6 or E7; 14/24 women with recent clearance had E6 and 8/24 had E7 response. Women with intermittent persistence behaved similar to clearance group than recent persistors: 50% were positive to E6 and 20% to E7. In summary, anti E6 responses appeared critical in the immediate control of HPV and in some women an immune tolerance eventually develops if HPV is not eliminated soon after infection.