Protective effects of broccoli (Brassica oleracea) and its active components against radical-induced oxidative damage.
Protective effects of broccoli (Brassica oleracea) and its active components against radical-induced oxidative damage.
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西兰花（Brassica oleracea）及其活性成分对自由基引起的氧化损伤的保护作用。
DOI：
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发表时间：
2005
影响因子：
1.6
通讯作者：
E. Cho
中科院分区：
文献类型：
--
作者：
X. Piao;H. Kim;T. Yokozawa;Young A Lee;X. Piao;E. Cho
研究了自由基生成物2，2‘-偶氮二(2-氨基丙烷)二盐酸盐(AAPH)对青花菜肾上皮LLC-PK1细胞的自由基清除作用和对氧化损伤的保护作用，并用生物测定联用的方法鉴定了其活性成分。青花菜的甲醇提取物、CH2Cl2、BuOH和H2O组分对1，1-二苯基-2-苦基肼(DPPH)的清除作用呈剂量依赖性。此外，它们对AAPH诱导的LLC-PK1细胞损伤具有保护作用，且呈剂量依赖性。其中，丁羟组分的活性最高，表明丁羟组分中含有具有抗氧化能力的活性成分。采用生测分离的方法，从丁羟组分中分离得到活性成分，鉴定为1，2-二异甘醇-2-阿魏酰龙胆二糖和1-芥子酰基-2-阿魏酰龙胆二糖。这两个西兰花提取物对DPPH自由基具有较强的抗氧化作用，其IC50值分别为5.18和7.52微克/毫升。此外，这些化合物显著且呈剂量依赖性地恢复了AAPH处理后降低的细胞存活率，表明它们具有保护细胞免受氧化损伤的作用。本研究表明，西兰花具有良好的抗氧化能力，西兰花中的羟基肉桂酸酯1，2-二异甘油基龙胆二糖和1-芥子酰基-2-阿魏酰龙胆二糖被认为是具有抗氧化作用的活性成分。
The radical scavenging effect and protective potential from oxidative damage by radical generator, 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH), in renal epithelial LLC-PK1 cell of broccoli (Brassica oleracea) were investigated and identified the active components under the bioassay-linked fractionation method. The MeOH extract, and fractions of CH2Cl2, BuOH and H2O from broccoli showed the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging effect in a dose-dependent manner. In addition, they exerted the protective effect against LLC-PK1 cellular damage induced by AAPH dose-dependently. In particular, the BuOH fraction was evaluated as the most active fraction, indicating that the BuOH fraction contains the active components with antioxidative capacity. Employing a bioassay-linked fractionation method, the active principles were isolated and characterized as 1,2-disinapoylgentiobiose and 1-sinapoyl-2-feruloylgentiobiose from the BuOH fraction. These two compounds from broccoli displayed potent antioxidant effects against the DPPH radical, showing the IC50 values of 5.18 and 7.52 microg/mL, respectively. Moreover, the compounds significantly and dose-dependently recovered cell viability lowered by AAPH treatment, suggesting the protective roles from cellular oxidative damage. The present study suggests that broccoli has excellent antioxidative potential and the hydroxycinamic acid esters from broccoli, 1,2-disinapoylgentiobiose and 1-sinapoyl-2-feruloylgentiobiose, are considered as the active components with antioxidative effect.
蔬菜、水果和谷物纤维的摄入量与男性患冠心病的风险。
DOI：
10.1001/jama.1996.03530300031036
发表时间：
1996
期刊：
JAMA
影响因子：
--
作者：
Rimm,EB;Ascherio,A;Giovannucci,E;Spiegelman,D;Stampfer,MJ;Willett,WC
通讯作者：
Willett,WC
纽约州布法罗饮食与癌症病例对照研究的结果。
影响因子：
11.2
作者：
Graham,S
通讯作者：
Graham,S