Gamma Irradiation as an Effective Method for Inactivation of Emerging Viral Pathogens
Gamma Irradiation as an Effective Method for Inactivation of Emerging Viral Pathogens
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DOI：
10.4269/ajtmh.18-0937
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发表时间：
2019-01-01
影响因子：
3.3
通讯作者：
Feldmann, Heinz
中科院分区：
文献类型：
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作者：
Feldmann, Friederike;Shupert, W. Lesley;Feldmann, Heinz
使用钴 - 60源进行伽马辐射是一种常用的方法，用于使传染性标本失活，以便在后续实验室操作中安全处理。在此，我们确定了辐射剂量，以安全地使含有不同新发/再发病毒病原体的液态蛋白质标本失活，这些病原体已知会导致被忽视的热带疾病以及具有区域或全球公共卫生重要性的其他疾病。通过使用具有代表性的沙粒病毒、布尼亚病毒、冠状病毒、丝状病毒、黄病毒、正粘病毒和副粘病毒，我们发现这些有包膜的病毒对辐射处理的敏感性不同，使每毫升1×10⁶ 50%组织培养感染剂量（TCID₅₀）的目标剂量失活的吸收剂量范围为1到5兆拉德。这一发现似乎通常与基因组大小呈负相关。我们的数据可能有助于指导其他机构测试和验证安全的失活程序。
Gamma irradiation using a cobalt-60 source is a commonly used method for the inactivation of infectious specimens to be handled safely in subsequent laboratory procedures. Here, we determined irradiation doses to safely inactivate liquid proteinaceous specimens harboring different emerging/reemerging viral pathogens known to cause neglected tropical and other diseases of regional or global public health importance. By using a representative arenavirus, bunyavirus, coronavirus, filovirus, flavivirus, orthomyxovirus, and paramyxovirus, we found that these enveloped viruses differed in their susceptibility to irradiation treatment with adsorbed doses for inactivation of a target dose of 1 x 10(6) 50% tissue culture infectious dose (TCID50)/mL ranging from 1 to 5 MRads. This finding seemed generally inversely correlated with genome size. Our data may help to guide other facilities in testing and verifying safe inactivation procedures.