Determination of ethambutol in plasma by high-performance liquid chromatography with fluorescence detection
Determination of ethambutol in plasma by high-performance liquid chromatography with fluorescence detection
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高效液相色谱荧光检测法测定血浆中乙胺丁醇
DOI：
10.2116/analsci.7.supple_949
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
S. Takitani
中科院分区：
文献类型：
--
作者：
Masao Suzuki;T. Ono;S. Takitani
建立了一种测定血浆中乙胺丁醇（EMB）的高效液相色谱方法。该方法包括用Extrelut - 1柱对血浆中的EMB进行净化，然后用9 - 芴基甲基氯甲酸酯对药物进行柱前荧光衍生化。EMB可在0.18 - 18 nmol/ml范围内测定，相对标准偏差为1.88 - 3.39%。该方法适用于单次注射EMB后兔血浆中EMB浓度的测定。
A high-performance liquid chromatographic method for determining ethambutol (EMB) in plasma has been developed. The method involves the clean-up of plasma EMB with an Extrelut-1 column followed by the pre-column fluorescence derivatization of the drug with 9-fluorenylmethylchloroformate. EMB can be determined in the range of 0.18-18nmol/ml, with a relative standard deviation of 1.88-3.39%. The method is applicable to the measurement of EMB concentration in rabbit plasma after single injection of EMB.