Modulation of Arabidopsis and monocot root architecture by CLAVATA3/EMBRYO SURROUNDING REGION 26 peptide.
Modulation of Arabidopsis and monocot root architecture by CLAVATA3/EMBRYO SURROUNDING REGION 26 peptide.
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DOI：
10.1093/jxb/erv360
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发表时间：
2015-08
影响因子：
6.9
通讯作者：
De Smet I
中科院分区：
文献类型：
--
作者：
Czyzewicz N;Shi CL;Vu LD;Van De Cotte B;Hodgman C;Butenko MA;De Smet I
CLE26 在调节拟南芥和单子叶植物根结构中发挥重要作用，并与生长素信号相互作用。植物根对于多种过程都很重要，包括养分和水分的吸收、锚定和机械支撑、储存功能，以及作为与土壤环境的主要界面。几种小信号肽和受体激酶已被证明会影响初生根的生长，但人们对它们在侧根发育中的作用知之甚少。在这种背景下，CLE 家族是本研究的重点，这是一组已被证明影响广泛发育过程的小信号肽。在此，探讨了几个 CLE 家族成员侧根起始过程中的表达模式，并评估了在根中显示特定表达模式的 CLE1、CLE4、CLE7、CLE26 和 CLE27 在多大程度上参与调节拟南芥根结构。使用化学合成的肽变体，发现 CLE26 在调节拟南芥根结构中发挥重要作用，并与生长素信号相互作用。此外，通过丙氨酸扫描和计算机结构建模，精确定位了 CLE26 肽序列中影响其活性的关键残基。最后，提出了有关 CLE26 在调节单子叶植物根结构中的作用的一些有趣的相似点和差异。
CLE26 plays an important role in regulating A. thaliana and monocot root architecture, and interacts with auxin signalling. Plant roots are important for a wide range of processes, including nutrient and water uptake, anchoring and mechanical support, storage functions, and as the major interface with the soil environment. Several small signalling peptides and receptor kinases have been shown to affect primary root growth, but very little is known about their role in lateral root development. In this context, the CLE family, a group of small signalling peptides that has been shown to affect a wide range of developmental processes, were the focus of this study. Here, the expression pattern during lateral root initiation for several CLE family members is explored and to what extent CLE1, CLE4, CLE7, CLE26, and CLE27, which show specific expression patterns in the root, are involved in regulating root architecture in Arabidopsis thaliana is assessed. Using chemically synthesized peptide variants, it was found that CLE26 plays an important role in regulating A. thaliana root architecture and interacts with auxin signalling. In addition, through alanine scanning and in silico structural modelling, key residues in the CLE26 peptide sequence that affect its activity are pinpointed. Finally, some interesting similarities and differences regarding the role of CLE26 in regulating monocot root architecture are presented.