Nurse bee behaviour manipulates worker honeybee (Apis mellifera L.) reproductive development
Nurse bee behaviour manipulates worker honeybee (Apis mellifera L.) reproductive development
复制标题
DOI：
10.1016/j.anbehav.2014.02.012
复制
发表时间：
2014-06-01
期刊：
影响因子：
2.5
通讯作者：
Page, Robert E., Jr.
中科院分区：
文献类型：
--
作者：
Wang, Ying;Kaftanoglu, Osman;Page, Robert E., Jr.
非生殖种姓的进化是进化生物学中的一个基本问题。意大利蜜蜂有生殖分工：蜂王是蜂群中的初级产蛋者，有200多个卵巢细丝(卵巢小管)，而工蜂通常不繁殖，卵巢小管不到20个。卵泡的数量影响工蜂的觅食行为，以及在蜂王不在的情况下产卵的倾向，这表明生殖调控网络是随着蜜蜂工蜂的觅食分工而演变的。哺乳蜜蜂的取食行为和幼虫发育规划的协同作用导致了蚁后和工蜂的分化，并导致了卵巢数、体重和觅食行为的变化。在这里，我们测试了哺乳蜜蜂如何影响工蜂的卵巢数和体重，以及幼虫在不同的幼虫生命阶段对食物输送的反应。我们的发现表明，护士通过控制食物传递来控制幼虫的生长和卵巢数，幼虫对食物的反应因幼虫的生活期和基因而不同。1~4龄(L1~L4)幼虫体质量对营养较为敏感，5龄(L5)幼虫体质量对营养更为敏感。总体而言，我们能够将蜜蜂的哺乳程序和幼虫发育程序脱钩。我们得出结论，L5期间的哺乳行为对于调节工人的卵巢数是至关重要的。(C)2014年动物行为研究协会。爱思唯尔有限公司出版。保留所有权利。
The evolution of nonreproductive castes is a fundamental question in evolution biology. The honeybee Apis mellifera L. has a reproductive division of labour: the queen is the primary egg-layer in a colony and has more than 200 ovarian filaments (ovarioles), whereas a worker normally does not reproduce and has fewer than 20 ovarioles. The number of ovarioles influences worker foraging behaviour and the propensity to become an egg-layer in the absence of the queen, suggesting that reproductive regulatory networks evolved with foraging division of labour in honeybee workers. Cooperation between nurse bee feeding behaviour and larval developmental programming results in the differentiation of queens and workers along with variation in ovariole number, body mass and foraging behaviour. Here, we tested how nurse bees affect ovariole number and body mass in workers, and how larvae respond to food delivery during different larval life stages. Our findings demonstrate that nurses control larvae growth and ovariole number by temporally manipulating food delivery and that the response of larvae to food differs with larval life stage and genotype. Body mass of larvae was more sensitive to nutrition during the first to the fourth instar (L1-L4), whereas ovariole number was more sensitive during the fifth instar (L5). Overall, we were able to decouple the nurse feeding program and the larval development program in honeybees. We conclude that nurse feeding behaviour during L5 is critical for modulating ovariole number in workers. (C) 2014 The Association for the Study of Animal Behaviour. Published by Elsevier Ltd. All rights reserved.