Effects of carbon-ion beam irradiation on the angiogenic response in lung adenocarcinoma A549 cells
Effects of carbon-ion beam irradiation on the angiogenic response in lung adenocarcinoma A549 cells
复制标题
碳离子束照射对肺腺癌A549细胞血管生成反应的影响
DOI：
10.1002/cbin.10327
复制
发表时间：
2014-11-01
影响因子：
3.9
通讯作者：
Wang, Yali
中科院分区：
文献类型：
--
作者：
Liu, Yuanyuan;Liu, Yang;Wang, Yali
放射治疗主要集中在杀死癌细胞，而很少关注支持肿瘤生长和转移的过程，包括血管生成过程。为探讨碳离子辐射对肺癌细胞血管生成的影响，我们检测了碳离子或X射线照射A549细胞的肿瘤条件培养液（TCM）中血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）的表达，以及TCM诱导的血管内皮细胞生长、侵袭和管腔形成。2、4Gy的碳离子照射对A549细胞TCM中血管内皮生长因子分泌无明显影响，而1Gy的照射则显著降低血管内皮生长因子和碱性成纤维细胞生长因子的水平。1Gy的碳离子照射抑制内皮细胞的侵袭和管形成。X射线照射的A549细胞的TCM促进血管生成，而碳离子照射的A549细胞的TCM在2或4Gy下没有影响。这些结果表明，在1Gy的碳离子辐射显着抑制血管生成的过程中，在体外通过抑制内皮细胞的侵袭和管的形成，这与血管内皮生长因子和碱性成纤维细胞生长因子的生产。
Radiotherapy has been focused mainly on killing cancer cells, and little attention has been paid to the process supporting tumor growth and metastasis, including the process of angiogenesis. To investigate the effects of carbon-ion irradiation on angiogenesis in lung cancer cells, we examined the expression of vascular endothelial growth factor and basic fibroblast growth factor in the tumor conditioned medium (TCM) of A549 cells exposed to carbon-ion or X-ray irradiation, as well as endothelial cell growth, invasion, and tube formation induced by TCM. No changes in vascular endothelial growth factor secretion were detected in the TCM of A549 cells exposed to carbon-ion irradiation at 2 or 4Gy, whereas 1Gy of irradiation significantly decreased vascular endothelial growth factor and basic fibroblast growth factor levels. Carbon-ion irradiation at 1Gy inhibited endothelial cell invasion and tube formation. The TCM from A549 cells irradiated with X-ray promoted angiogenesis, whereas the TCM of A549 cells exposed to carbon-ion irradiation at 2 or 4Gy had no effect. These findings suggest that carbon-ion irradiation at 1Gy significantly suppressed the process of angiogenesis in vitro by inhibiting endothelial cell invasion and tube formation, which are related to vascular endothelial growth factor and basic fibroblast growth factor production.