RNA-based diagnosis in a multicellular specimen by whole mount in situ hybridization using an RNA-specific probe.
RNA-based diagnosis in a multicellular specimen by whole mount in situ hybridization using an RNA-specific probe.
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DOI：
10.1016/j.bmc.2012.08.028
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发表时间：
2012-10
影响因子：
3.5
通讯作者：
T. Ueda;A. Kobori;A. Yamayoshi;H. Yoshida;M. Yamaguchi;A. Murakami
中科院分区：
文献类型：
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作者：
T. Ueda;A. Kobori;A. Yamayoshi;H. Yoshida;M. Yamaguchi;A. Murakami
最近的RNA研究揭示了各种RNA在细胞功能中的密切参与。rna正成为研究基因表达细节的必然目标分子。RNA及其相关复合物也有望成为疾病诊断的靶点。多细胞标本，如器官组织、组织病理学标本和胚胎是基于rna的诊断技术的可能目标。在本报告中，我们着重于提供这种时空信息的方法。我们证明了一种rna特异性探针（OMUpy2）不仅可以在时间和空间上检测果蝇胚胎中的特定mRNA，而且相对快速和易于使用。该探针OMUpy2可应用于其他多细胞系统，用于基于rna的诊断和研究。这篇论文令人鼓舞的结果显示了基于rna的检测在生物学研究和诊断医学方面的巨大潜力。
Recent RNA research has revealed the close involvement of various RNAs in cellular functions. RNAs are becoming the inevitable target molecules for research into details of gene expression. RNA and its related complexes are also promising targets for disease diagnosis. Multi cellular specimens such as organ tissues, histopathological specimens, and embryos are among the possible targets of RNA-based diagnostic techniques. In this report, we focused on a method that would provide such spatial and temporal information. We demonstrated that an RNA-specific probe (OMUpy2) was not only applicable to the detection of a specific mRNA in Drosophila embryos in a temporal and spatial manner but was also relatively quick and easy to use. The probe, OMUpy2, could be applied to other multi cellular systems for RNA-based diagnosis and research. The promising results of this manuscript show the great potential of RNA-based detection for both biological research and diagnostic medicine.