L-Serine and glycine serve as major astroglia-derived trophic factors for cerebellar Purkinje neurons
L-Serine and glycine serve as major astroglia-derived trophic factors for cerebellar Purkinje neurons
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DOI：
10.1073/pnas.200364497
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发表时间：
2000-10-10
影响因子：
11.1
通讯作者：
Hirabayashi, Y
中科院分区：
文献类型：
--
作者：
Furuya, S;Tabata, T;Hirabayashi, Y
神经胶质细胞通过提供营养因子支持中枢神经元的存活和发育。在这里，我们证明，L-丝氨酸（L-Ser）和甘氨酸（Cly）也是胶质源性营养因子。这些氨基酸由星形胶质细胞释放并促进培养的小脑浦肯野神经元的存活、树突发生和电生理发育。虽然L-ser和Gly通常被归类为非必需氨基酸，但其生物合成的关键酶3-磷酸甘油酸脱氢酶（3 PGDH）在浦肯野神经元中不表达。与此相反，Bergman胶质细胞，一种小脑皮质中的天然星形胶质细胞，高表达3 PGDH。这些数据表明，L-Ser和Gly介导胶质细胞对浦肯野神经元的营养作用。
Glial cells support the survival and development of central neurons through the supply of trophic factors. Here we demonstrate that L-serine (L-Ser) and glycine (Cly) also are glia-derived trophic factors. These amino acids are released by astroglial cells and promote the survival, dendritogenesis, and electrophysiological development of cultured cerebellar Purkinje neurons. Although L-ser and Gly are generally classified as nonessential amino acids, 3-phosphoglycerate dehydrogenase (3PGDH), a key enzyme for their biosynthesis, is not expressed in Purkinje neurons. By contrast, the Bergman glia, a native astroglia in the cerebellar cortex, highly expresses 3PGDH, These data suggest that L-Ser and Gly mediate the trophic actions of glial cells on Purkinje neurons.