The cold response regulator CBF1 promotes Arabidopsis hypocotyl growth at ambient temperatures
The cold response regulator CBF1 promotes Arabidopsis hypocotyl growth at ambient temperatures
复制标题
冷反应调节因子CBF1促进拟南芥下胚轴在环境温度下的生长
DOI：
10.15252/embj.2019103630
复制
发表时间：
2020-05-25
期刊：
影响因子：
11.4
通讯作者：
Li, Jigang
中科院分区：
文献类型：
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作者：
Dong, Xiaojing;Yan, Yan;Li, Jigang
光和温度是协调调节植物生长和存活的两个核心环境因子。然而，在植物中整合光和温度信号通路的机制仍然知之甚少。在这里，我们报告CBF 1，一个AP 2/ERF家族转录因子对植物冷驯化至关重要，促进拟南芥下胚轴在环境温度下的生长。我们发现CBF 1增加了PIF 4和PIF 5的蛋白质丰度，这两种光敏色素相互作用的bHLH家族转录因子在调节植物生长和发育中起着关键作用，通过直接结合到它们的启动子以诱导它们的基因表达，并通过抑制它们与phyB在光照下的相互作用。此外，我们的数据表明，CBF 1在22°C和17°C下促进PIF 4/PIF 5蛋白积累和下胚轴生长，但在4°C下不促进，在17°C下的作用比在22°C下更突出。总之，我们的研究表明CBF 1通过促进光中PIF 4和PIF 5蛋白的丰度来整合下胚轴生长的光和温度控制，从而为植物中光和温度信号通路的整合机制提供了见解。
Light and temperature are two core environmental factors that coordinately regulate plant growth and survival throughout their entire life cycle. However, the mechanisms integrating light and temperature signaling pathways in plants remain poorly understood. Here, we report that CBF1, an AP2/ERF‐family transcription factor essential for plant cold acclimation, promotes hypocotyl growth under ambient temperatures in Arabidopsis. We show that CBF1 increases the protein abundance of PIF4 and PIF5, two phytochrome‐interacting bHLH‐family transcription factors that play pivotal roles in modulating plant growth and development, by directly binding to their promoters to induce their gene expression, and by inhibiting their interaction with phyB in the light. Moreover, our data demonstrate that CBF1 promotes PIF4/PIF5 protein accumulation and hypocotyl growth at both 22°C and 17°C, but not at 4°C, with a more prominent role at 17°C than at 22°C. Together, our study reveals that CBF1 integrates light and temperature control of hypocotyl growth by promoting PIF4 and PIF5 protein abundance in the light, thus providing insights into the integration mechanisms of light and temperature signaling pathways in plants.