METHODS FOR MICROCARRIER CULTURE OF BOVINE PULMONARY-ARTERY ENDOTHELIAL-CELLS AVOIDING THE USE OF ENZYMES
METHODS FOR MICROCARRIER CULTURE OF BOVINE PULMONARY-ARTERY ENDOTHELIAL-CELLS AVOIDING THE USE OF ENZYMES
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DOI：
10.1016/0040-8166(80)90017-8
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发表时间：
1980-01-01
期刊：
影响因子：
2.6
通讯作者：
WHITAKER, C
中科院分区：
文献类型：
--
作者：
RYAN, US;MORTARA, M;WHITAKER, C
位于肺内皮细胞管腔表面的酶与循环溶质相互作用，特别是与血管活性物质相互作用，调节全身动脉血的激素组成。发生在内皮细胞表面或其附近的反应的范围和复杂性显然比以前认识到的要大。用于确定特定内皮功能的细胞培养的质量必须仔细控制，同时对肺内皮细胞长期培养的影响有更深入的了解。开发了肺内皮细胞培养的新技术，在分离步骤和传代培养过程中避免暴露于蛋白水解酶。机械收获和微载体珠培养的结合为肺内皮细胞的长期、大规模培养提供了一个系统，其特点在很大程度上决定了可以进行的生化研究的范围。
Enzymes situated along the luminal surface of pulmonary endothelial cells interact with circulating solutes, notably with vasoactive substances, to regulate the hormonal composition of systemic arterial blood. The range and complexity of reactions occurring at or near the surface of endothelial cells apparently are greater than previously recognized. The quality of cell cultures used to define specific endothelial functions must be carefully controlled, together with development of improved understanding of the effects of long-term culture of pulmonary endothelial cells. New techniques were developed for the culture of pulmonary endothelial cells which avoid exposure to proteolytic enzymes at the isolation step and during subculture. A combination of mechanical harvest and culture on microcarrier beads provided a system for the long-term, large-scale culture of pulmonary endothelial cells, features which to a large extent determine the scope of biochemical studies which can be undertaken.