Identification and characterization of two plasma membrane aquaporins in durum wheat (Triticum turgidum L. subsp. durum) and their role in abiotic stress tolerance
Identification and characterization of two plasma membrane aquaporins in durum wheat (Triticum turgidum L. subsp. durum) and their role in abiotic stress tolerance
复制标题
DOI：
10.1016/j.plaphy.2011.06.002
复制
发表时间：
2011-09-01
影响因子：
6.5
通讯作者：
Masmoudi, Khaled
中科院分区：
文献类型：
--
作者：
Ayadi, Malika;Cavez, Damien;Masmoudi, Khaled
植物质膜内在蛋白（PIP）在异种卵母细胞中表达时具有不同的水通道活性，可分为两个系统发育组，即PIP 1和PIP 2。PIP 2诱导膜渗透水渗透系数（P-f）的显着增加，而PIP 1通常是无活性的。本文报道了两个硬粒小麦（Triticum turgidum L subsp. durum）cDNA编码分别属于PIP 1和PIP 2亚家族的TdPIP 1;1和TdPIP 2;1。与TdPIP 1;1相反，TdPIP 2;1在爪蟾卵母细胞中的表达导致与注水卵母细胞相比P-f增加。与仅表达TdPIP 2;1的卵母细胞相比，非功能性TdPIP 1;1和功能性TdPIP 2;1的共表达导致P-f显著增加。缺失前两个跨膜结构域的TdPIP 2;1的截短形式，tdPIP 2;1，没有水通道活性。然而，它与功能性TdPIP 2：1的共表达部分抑制P-f并破坏植物水通道蛋白的活性。与非洲爪蟾卵母细胞中开发的方法相反，表达TdPIP 1; 1或TdPIP 2;1的转基因烟草植物的表型分析产生了对渗透胁迫和盐胁迫的耐受表型。TdPIP 1：1和TdPIP 2：1在盐胁迫和脱落酸的挑战下，在耐盐小麦品种的根和叶中差异调节。共聚焦显微镜分析表达TdPIP 1：1和TdPIP 2;1融合到绿色荧光蛋白的烟草根表明，蛋白质定位于质膜。(C)2011年Elsevier Masson SAS。All rights reserved.
Plant plasma membrane intrinsic proteins (PIP) cluster in two phylogenetic groups, PIP1 and PIP2 that have different water channel activities when expressed in Xeno pus oocytes. PIP2s induce a marked increase of the membrane osmotic water-permeability coefficient (P-f), whereas PIP1s are generally inactive. Here we report the cloning of two durum wheat (Triticum turgidum L subsp. durum) cDNAs encoding TdPIP1;1 and TdPIP2;1 belonging to the PIP1 and PIP2 subfamilies, respectively. Contrary to TdPIP1;1, expression of TdPIP2;1 in Xenopus oocytes resulted in an increase in P-f compared to water-injected oocytes. Co-expression of the non-functional TdPIP1;1 and the functional TdPIP2;1 lead to a significant increase in P-f compared with oocytes expressing TdPIP2;1 alone. A truncated form of TdPIP2;1, tdpip2;1, missing the first two transmembrane domains, had no water channel activity. Nonetheless, its co-expression with the functional TdPIP2:1 partially inhibits the P-f and disrupt the activities of plant aquaporins. In contrast to the approach developed in Xenopus oocytes, phenotypic analyses of transgenic tobacco plants expressing TdPIP1; 1 or TdPIP2;1 generated a tolerance phenotype towards osmotic and salinity stress. TdPIP1:1 and TdPIP2:1 are differentially regulated in roots and leaves in the salt-tolerant wheat variety when challenged with salt stress and abscisic acid. Confocal microscopy analysis of tobacco roots expressing TdPIP1:1 and TdPIP2;1 fused to the green fluorescent protein showed that the proteins were localized at the plasma membrane. (C) 2011 Elsevier Masson SAS. All rights reserved.