Immunohistochemical staining for androgen receptors - A sensitive marker of sebaceous differentiation
Immunohistochemical staining for androgen receptors - A sensitive marker of sebaceous differentiation
复制标题
DOI：
10.1097/00000372-199910000-00004
复制
发表时间：
1999-10-01
影响因子：
1.1
通讯作者：
Smoller, B
中科院分区：
文献类型：
--
作者：
Bayer-Garner, IB;Givens, V;Smoller, B
雄激素受体（AR）存在于正常皮肤中，定位于皮脂腺的基底细胞和分化细胞，因此，皮脂腺是雄激素敏感组织。雄激素受体表达在43例皮脂腺肿瘤中进行了检测，包括8例皮脂腺癌，22例皮脂腺腺瘤，12例皮脂腺增生，1例皮脂腺上皮瘤，以及14例鳞状细胞癌，2例透明细胞棘皮瘤和35例基底细胞癌。在所有的皮脂腺肿瘤中也检测了上皮膜抗原（EMA）的表达。所有标本均用福尔马林固定，石蜡包埋。在所有皮脂腺肿瘤样本中观察到弥漫性阳性核雄激素受体抗体免疫组化染色，而大约60%的基底细胞癌仅显示核雄激素受体免疫反应性局灶性阳性。透明细胞棘皮瘤和鳞状细胞癌均为阴性。而所有的皮脂腺肿瘤表现出雄激素受体的免疫反应性，染色模式更显着的细胞核的皮脂腺细胞和分化的皮脂腺细胞的腺瘤，增生，上皮瘤病变比在细胞核的低分化皮脂腺癌细胞。除皮脂腺癌外，所有皮脂腺肿瘤均呈EMA免疫反应阳性。在皮脂腺癌中，大多数珍珠样分化的标本中EMA染色阴性，但随着分化程度的增加，癌对EMA的免疫反应逐渐增强。我们已经证明，包括皮脂腺癌在内的皮脂腺肿瘤细胞核显示雄激素受体（AR）的免疫反应性，AR的免疫组化染色可能是皮脂腺分化的可靠标志物，AR可能是比EMA更好的皮脂腺分化标志物，特别是在低分化的皮脂腺癌中。
Androgen receptors (AR) are present in normal skin being localized to the basal and differentiating cells of the sebaceous gland, and as such, sebaceous glands art: androgen sensitive tissue. Androgen receptor expression was examined in 43 sebaceous neoplasms including 8 sebaceous carcinomas, 22 sebaceous adenomas, 12 specimens showing sebaceous hyperplasia, and 1 sebaceous epithelioma, as well as in 14 squamous cell carcinomas, 2 clear cell acanthomas, and 35 basal cell carcinomas. Epithelial membrane antigen (EMA) expression was also examined in all of the sebaceous neoplasms. All specimens were fixed in formalin and embedded in paraffin. Diffuse positive nuclear androgen receptor antibody immunohistochemical staining was observed in all samples of sebaceous neoplasms, whereas approximately 60% of basal cell carcinomas showed only focal positivity for nuclear androgen receptor immunoreactivity. Clear cell acanthomas and squamous cell carcinomas were uniformly negative. Whereas all sebaceous neoplasms exhibited immunoreactivity for androgen receptors, the staining pattern was more marked in the nuclei of seboblasts and differentiating sebocytes in the adenomatous, hyperplastic, and epitheliomatous lesions than in the nuclei of the less differentiated sebaceous carcinoma cells. Al the sebaceous neoplasms except for sebaceous carcinomas exhibited immunoreactivity for EMA, In the sebaceous carcinomas, EMA staining was absent in the most pearly differentiated specimen, but with increasing differentiation, the carcinomas became immunoreactive to EMA. We have shown that the nuclei of sebaceous neoplasms, including sebaceous gland carcinomas, show immunoreactivity for androgen receptors (AR), that immunohistochemical staining for the presence of AR may be a reliable marker of sebaceous differentiation, and that the AR may be a better marker of sebaceous differentiation than EMA, particularly in poorly differentiated sebaceous carcinomas.