Production of highly stable spray dried phage formulations for treatment of Pseudomonas aeruginosa lung infection.
Production of highly stable spray dried phage formulations for treatment of Pseudomonas aeruginosa lung infection.
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DOI：
10.1016/j.ejpb.2017.09.002
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发表时间：
2017-12
期刊：
影响因子：
--
通讯作者：
Chan HK
中科院分区：
文献类型：
--
作者：
Chang RY;Wong J;Mathai A;Morales S;Kutter E;Britton W;Li J;Chan HK
噬菌体疗法用于治疗由耐药性细菌引起的肺部感染的潜力已得到充分认可。本研究的目的是研究赋形剂对形态上不同的PEV-PEV短尾病毒和PEV肌病毒的喷雾干燥粉末的稳定和雾化的影响。针对90种细菌宿主的临床菌株筛选了7种抗假单胞菌抗生素，并基于宿主范围选择了3种抗生素用于制剂研究。利用实验设计来评估不同赋形剂对喷雾干燥片剂的稳定化和雾化的影响。在含有海藻糖或乳糖和亮氨酸作为赋形剂的喷雾干燥制剂中，短尾病毒和肌病毒均稳定，喷雾干燥期间滴度降低小于1-log 10，乳糖提供优于海藻糖的上级噬菌体保护。此外，喷雾干燥的噬菌体制剂以85 L/min分散在Osmohaler中产生超过50%的高细颗粒分数。结果表明，本研究中的噬菌体可以使用相同的赋形剂组成形成可吸入干粉噬菌体制剂。喷雾干燥的各种类型的溶菌制剂对肺部感染的治疗具有显著的潜力。
The potential of bacteriophage therapy for the treatment of pulmonary infections caused by antibiotic-resistant bacteria has been well recognised. The purpose of this study was to investigate the effect of excipients on stabilisation and aerosolisation of spray dried powders of morphologically different phages – PEV podovirus and PEV myovirus. Seven anti-pseudomonal phages were screened against 90 clinical strains of bacterial hosts and three of the phages were selected for formulation study based on the host range. Design of experiments was utilised to assess the effect of different excipients on the stabilisation and aerosolisation of spray dried phages. Both podovirus and myovirus phages were stable in spray dried formulations containing trehalose or lactose and leucine as excipients with less than 1-log10 titre reduction during spray drying, with lactose providing superior phage protection over trehalose. Furthermore, the spray dried phage formulations dispersed in an Osmohaler at 85 L/min produced a high fine particle fraction of over 50 %. The results showed that the phages in this study can form respirable dry powder phage formulations using the same excipient composition. Spray dried various types of lytic phages hold significant potential for the treatment of pulmonary infections.
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影响因子：
5.8
作者：
Ajmer, Ankur;Scherliess, Regina
通讯作者：
Scherliess, Regina
影响因子：
3.7
作者：
Chew, NYK;Tang, P;Raper, JA
通讯作者：
Raper, JA
影响因子：
2.4
作者：
Cooper, C. J.;Denyer, S. P.;Maillard, J. -Y.
通讯作者：
Maillard, J. -Y.
DOI：
10.1089/jamp.2008.0701
发表时间：
2008-12-01
影响因子：
3.4
作者：
Golshahi, Laleh;Seed, Kimberley D.;Finlay, Warren. H.
通讯作者：
Finlay, Warren. H.
影响因子：
3.8
作者：
Matinkhoo, Sadaf;Lynch, Karlene H.;Vehring, Reinhard
通讯作者：
Vehring, Reinhard