A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos
A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos
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DOI：
10.3390/cells9040898
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发表时间：
2020-04-01
期刊：
影响因子：
6
通讯作者：
Roch, Fernando
中科院分区：
文献类型：
--
作者：
Feizbakhsh, Omid;Pontheaux, Florian;Roch, Fernando
海胆胚胎为分析细胞周期进程和有丝分裂的分子机制提供了一个有价值的系统。然而，尽管已知翻译后修饰对组蛋白活性的调节在细胞分裂过程中起着重要作用，但这些修饰的动力学和影响尚未在发育中的胚胎中详细描述。利用不同的免疫检测技术，我们发现组蛋白3在苏氨酸3处的磷酸化水平与粒球藻早期胚胎的有丝分裂同步振荡。此外，我们还介绍了一项药理学研究的结果，该研究旨在分析这种关键的组蛋白翻译后修饰在海胆早期发育中的作用。
The sea urchin embryo provides a valuable system to analyse the molecular mechanisms orchestrating cell cycle progression and mitosis in a developmental context. However, although it is known that the regulation of histone activity by post-translational modification plays an important role during cell division, the dynamics and the impact of these modifications have not been characterised in detail in a developing embryo. Using different immuno-detection techniques, we show that the levels of Histone 3 phosphorylation at Threonine 3 oscillate in synchrony with mitosis in Sphaerechinus granularis early embryos. We present, in addition, the results of a pharmacological study aimed at analysing the role of this key histone post-translational modification during sea urchin early development.