Patterns of LHbetacf among women in health and disease.
Patterns of LHbetacf among women in health and disease.
复制标题
健康和疾病女性中 LHbetacf 的模式。
DOI：
10.1016/j.mce.2006.02.020
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发表时间：
2007
影响因子：
4.1
通讯作者：
Taliadouros,George
中科院分区：
文献类型：
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作者：
Birken,Steven;McChesney,Ruth;Yershova,Oksana;Gaughan,John;Pettersson,Kim;Rechenberg,Geoff;Wu,ChungH;Taliadouros,George
尿促性腺激素测定提供了一种分析激素分泌模式的实用方法。虽然研究方案已经揭示了血液中促性腺激素（如LH）的脉冲模式，但这些测定的临床用途有限，因为在研究环境之外，每日静脉穿刺取样是不可行的。然而，采集多份尿液样本提供了一种方法，可以使用一种方便且普遍适用的技术，基于对高度稳定的hLH β cf（用于监测LH）和hCG β cf（用于监测垂体hCG）的分析，实现患者促性腺激素模式的相同可视化。我们证明，两种不同的采样技术分析这些促性腺激素代谢产物产生了相同的信息，其排泄模式，无论是采样点尿或收集第一个早晨的排尿数天。接下来，我们研究了几个人群的核心排泄模式：来自普通人群的绝经期和绝经后妇女，以及来自生育中心的两个妇女人群，其中一个患有多囊卵巢（PCO）。PCO人群还被细分为有和无胰岛素抵抗（IR）的人群。我们发现，我们的hLH β cf试验不能测量LH变体（v-LH）纯合子受试者产生的LH核心（v-hLH β cf）的形式。我们检测的患者中没有一个是LH变异型纯合子。大多数非PCO患者hLH β cf峰值持续7-9天，而PCO患者hLH β cf峰值持续时间多在7天以下，且呈不稳定型。通过光谱统计方法证实了PCO和NPCO患者之间模式的总体差异。某些特征性hLH β cf模式的患病率在临床表现更严重的PCO女性中可能更高。使用尿促性腺激素代谢物分析，特别是hLH β cf，可以用更灵敏的生化测量补充主观超声检查。
Urine based gonadotropin assays provide a practical means of analyzing hormone secretion patterns. While research protocols have revealed pulsatile patterns of gonadotropins such as LH in the blood, these assays are of limited clinical use since daily venipuncture sampling is not feasible outside of a research environment. However, collection of several urine samples provides a method to achieve the same visualization of gonadotropin patterns in patients using a convenient and generally applicable technique based on analysis of the highly stable hLHβcf for monitoring LH and hCGβcf for monitoring pituitary hCG. We demonstrated that two different sampling techniques for analyzing these gonadotropin metabolites yielded the same information on their excretory patterns, either sampling of spot urines or collecting first morning void urines for several days. Next, we studied the core excretory patterns in several populations: menstruating and postmenopausal women from the general population, and two populations of women from a fertility center, one of which had polycystic ovaries (PCO). The PCO population was also subdivided into those with and without insulin resistance (IR). It was found that our hLHβcf assay did not measure the form of the LH core (v-hLHβcf) produced in subjects who were homozygous for a variant form of LH (v-LH). None of our patients tested were homozygous for the variant form of LH. It was also found that in most non-PCO (NPCO) patients, the hLHβcf peak lasted for 7–9 days while among the PCO patients this peak frequently lasted for less than 7 days and an erratic pattern tended to appear. The overall differences in patterns between the PCO and NPCO patients were confirmed by spectral statistical methods. The prevalence of certain characteristic hLHβcf patterns may be higher among women with PCO with a more severe clinical presentation. Use of urinary analysis of gonadotropin metabolites, especially hLHβcf, may supplement subjective ultrasound studies with more sensitive biochemical measurements.