The role of vascular endothelial growth inhibitor in wound healing.
The role of vascular endothelial growth inhibitor in wound healing.
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DOI：
10.1111/j.1742-481x.2006.00295.x
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发表时间：
2007-03-01
影响因子：
3.1
通讯作者：
Jiang, Wen G
中科院分区：
文献类型：
--
作者：
Conway, Kevin P;Price, Patricia;Jiang, Wen G
血管内皮生长抑制因子（VEGI）是一种内皮细胞特异性细胞因子，是内皮细胞增殖和血管生成的有效抑制剂。VEGI在与组织修复相关的血管生成中的作用以前没有被研究过。通过免疫组织化学和定量聚合酶链反应分别分析了不同伤口类型的活检组织中VEGI蛋白和转录物的存在。用VEGI表达质粒转染人血管内皮细胞系，检测其体外血管生成特性。VEGI的免疫组化染色显示，与慢性伤口或正常皮肤相比，急性伤口真皮层中的表达减少。VEGI通过体外测定阻止血管生成的能力表明，VEGI作为内皮细胞增殖和微管形成的抑制剂，并且加入肝细胞生长因子对表达VEGI基因的细胞系增加微管形成的能力几乎没有影响。VEGI在不同伤口类型中的异常表达似乎与这些伤口的愈合结果有关。慢性伤口中VEGI表达的改变构成了未来治疗的重要靶点。
Vascular endothelial growth inhibitor (VEGI) is an endothelial cell-specific cytokine and a potent inhibitor of endothelial cell proliferation and angiogenesis. The role of VEGI in angiogenesis related to tissue repair has previously not been investigated. Biopsies from different wound types were analysed by immunohistochemistry and quantitative polymerase chain reaction for the presence of VEGI protein and transcript, respectively. Human vascular endothelial cell line was transfected with VEGI expression plasmid and tested for their in vitro angiogenesis properties. Immunohistochemical staining for VEGI showed reduced expression in the dermal layer of the acute wounds compared with the chronic wound or normal skin. The ability of VEGI to prevent angiogenesis by in vitro assays showed that VEGI acts as a suppressor to the proliferation and microtubule formation of endothelial cells, and the addition of Hepatocyte Growth Factor had little effect on the ability of cell lines expressing the VEGI gene to increase microtubule formation. The aberrant expression of VEGI in different wound types appears to be linked to the outcome of the healing in these wounds. The altered expression of VEGI in chronic wounds constitutes an important target of future therapies.