Lipopolysaccharide-induced up-regulation of activated macrophages in the degenerating taste system
Lipopolysaccharide-induced up-regulation of activated macrophages in the degenerating taste system
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DOI：
10.1002/jnr.20438
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发表时间：
2005-04-01
影响因子：
4.2
通讯作者：
McCluskey, LP
中科院分区：
文献类型：
--
作者：
Cavallin, MA;McCluskey, LP
单侧鼓室索（CT）神经切除和维持限钠饮食导致对侧完整CT神经对钠的神经生理味觉反应迅速下降。脂多糖（LPS, 100 μ g / p）对免疫功能的上调诱导了正常钠味觉反应的恢复，表明缺钠饮食具有免疫抑制作用。事实上，在接受CT神经切片的对照组大鼠中，双侧舌侧活化的巨噬细胞数量增加，而在切片后缺钠大鼠中则没有这种情况。在目前的研究中，我们假设lps诱导的钠缺乏大鼠正常味觉功能的恢复是基于活化的巨噬细胞对去神经控制的反应增加。大鼠接受单侧CT神经切片，限钠饮食，和/或注射LPS (100 μ g; i.p.)，在切片后第2天过量使用戊巴比妥，并迅速解剖和冷冻舌头。然后对冷冻切片进行免疫组织化学染色，以确定活化巨噬细胞ED1染色的百分比或α β或γ δ T细胞的数量。在单侧CT神经切片后接受LPS的限钠大鼠中，活化的巨噬细胞水平显著增加，支持我们的假设。这些新发现表明LPS克服了限钠饮食引起的免疫抑制，也表明免疫系统在神经损伤后的味觉功能调节中起作用。(c) 2005 Wiley-Liss, Inc。
Unilateral chorda tympani (CT) nerve section and maintenance on a sodium-restricted diet leads to a rapid decrease in neurophysiological taste responses to sodium in the contralateral, intact CT nerve. Up-regulation of immune function with lipopolysaccharide (LPS; 100 mu g i.p.) induces a recovery of normal sodium taste responses, suggesting that the sodium-deficient diet is immunosuppressive. In fact, there is a bilateral increase in the number of lingual, activated macrophages in control-fed rats receiving CT nerve section that does not occur in sodium-deficient rats after sectioning. In the current study, we hypothesized that the LPS-induced recovery of normal taste function in sodium-deficient rats is based on an increase in the activated macrophage response to denervation. Rats receiving a unilateral CT nerve section, a sodium-restricted diet, and/or an injection of LPS (100 mu g; i.p.) were overdosed with pentobarbital at day 2 postsectioning, and tongues were rapidly dissected and frozen. Cryosections were then immunohistochemically stained to determine the percentage of ED1 staining for activated macrophages or the number of alpha beta or gamma delta T cells. Activated macrophage levels were significantly increased in sodium-restricted rats that received LPS following unilateral CT nerve section, supporting our hypothesis. These novel findings suggest that LPS overcomes the immunosuppression induced by the sodium-restricted diet and also indicate that the immune system plays a role in regulating taste function after neural injury. (c) 2005 Wiley-Liss, Inc.