EFFECTS OF ENZYME CONCENTRATION AND FILM THICKNESS ON THE ANALYTICAL PERFORMANCE OF A POLYPYRROLE GLUCOSE-OXIDASE BIOSENSOR
EFFECTS OF ENZYME CONCENTRATION AND FILM THICKNESS ON THE ANALYTICAL PERFORMANCE OF A POLYPYRROLE GLUCOSE-OXIDASE BIOSENSOR
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DOI：
10.1080/00032719508002676
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发表时间：
1995-01-01
影响因子：
2
通讯作者：
KIM, HS
中科院分区：
文献类型：
--
作者：
SHIN, MC;KIM, HS
我们描述了电聚合过程中的制备条件（例如沉积溶液中的酶浓度和膜厚度）对所得聚吡咯/葡萄糖氧化酶（PPy/GOD）膜的分析性能的影响。对所得 PPy/GOD 生物传感器对葡萄糖和抗坏血酸的响应进行了表征和优化。结果发现，酶浓度和膜厚度对所得生物传感器的响应具有相同的影响，并且存在这两个参数的多个最佳组。关于响应特性和干扰抑制的研究清楚地表明，在低酶浓度下使膜变厚可以显着提高生物传感器的性能。
We describe the effect of preparation conditions during electropolymerization such as enzyme concentration in the deposition solution and film thickness on the analytical performance of the resulting polypyrrole / glucose oxidase (PPy/GOD) film. The responses of the resulting PPy/GOD biosensor to glucose and ascorbate were characterized and optimized. It was found that the enzyme concentration and the film thickness have the equivalent effects on the response of the resulting biosensor and that several optimal sets of the two parameters exist. The investigations regarding the response characteristic and the suppression of interference clearly indicate that the performance of biosensor could be significantly improved by making the film thick at low enzyme concentration.