THE EFFECT OF ZINC ON MICROBIAL-GROWTH AND BACTERIAL KILLING BY CEFAZOLIN IN A STAPHYLOCOCCUS-AUREUS ABSCESS MILIEU
THE EFFECT OF ZINC ON MICROBIAL-GROWTH AND BACTERIAL KILLING BY CEFAZOLIN IN A STAPHYLOCOCCUS-AUREUS ABSCESS MILIEU
复制标题
DOI：
10.1093/infdis/168.4.893
复制
发表时间：
1993-10-01
影响因子：
6.4
通讯作者：
SUVARNA, PR
中科院分区：
文献类型：
--
作者：
BAMBERGER, DM;HERNDON, BL;SUVARNA, PR
脓肿中的微生物生长和抗菌杀菌作用都会减弱。据推测，脓肿中的锌缺乏，可能继发于一种类似钙保护素的中性粒细胞蛋白，可能是这些影响的部分原因。在兔组织笼脓肿模型中，脓肿上清液中锌的浓度为1.53微米。添加41.7mU的锌对金黄色葡萄球菌的生长和血清中头孢唑林的杀菌作用均无影响。在脓液上清液中，添加锌可促进未加抗生素的细菌生长和头孢唑林对细菌的杀灭。用超滤膜对脓液进行分级，结果表明，在30 kDa到50 kDa的范围内，这些效应可以重现。这些发现表明，脓液上清液中的一种类似于中性粒细胞蛋白钙保护素的蛋白质，可能通过其锌结合效应抑制脓肿内的微生物生长，但也抑制杀菌抗生素的活性。
Microbial growth and antimicrobial bacterial killing are both diminished in abscesses. It was postulated that zinc depletion in abscesses, perhaps secondary to a neutrophil protein resembling calprotectin, may be partly responsible for these effects. In a rabbit tissue-cage abscess model, pooled abscess supernatant concentration of zinc was < 1.53 muM. The addition of 41.7 muM zinc had no effect on Staphylococcus aureus growth or the bacterial killing effect of cefazolin in serum. In abscess fluid supernatants, bacterial growth without antibiotic and bacterial killing by cefazolin were both enhanced by the addition of zinc. Fractionation of the abscess fluid with ultrafiltration membranes showed that these effects could be reproduced with the fraction between 30 and 50 kDa. These findings suggest that a protein in abscess fluid supernatants that resembles the neutrophil protein calprotectin may, through its zinc binding effects, inhibit microbial growth within an abscess but also inhibit the activity of bactericidal antibiotics.