Subhypnotic doses of propofol accelerate extinction of conditioned taste aversion
Subhypnotic doses of propofol accelerate extinction of conditioned taste aversion
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DOI：
10.1016/s0166-4328(02)00375-3
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发表时间：
2003-05
影响因子：
2.7
通讯作者：
S. Ishitobi;T. Miyamoto;K. Oi;K. Toda
中科院分区：
文献类型：
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作者：
S. Ishitobi;T. Miyamoto;K. Oi;K. Toda
亚催眠剂量的异丙酚加速条件性味觉厌恶的消退。包括异丙酚在内的一些静脉麻醉剂已知会引起顺行性和逆行性遗忘。我们评估了异丙酚是否影响条件性味觉厌恶（CTA）范式形成的长期记忆。在整个实验过程中，允许大鼠4小时接触水。预处理饮水后，给予0.1%糖精钠（Sac）作为条件刺激（CS）20 min。腹膜内（i. p.）暴露于Sac后10分钟注射几种浓度（0.5- 100 mg/kg）的丙泊酚，随后在暴露于CS后30分钟腹腔注射0.15M LiCl（体重的2%）作为非条件刺激（US）。连续4天测定20 min内囊袋的摄入量。通过每种CS-US范式获得CTA的大鼠在条件化后的第一个测试日强烈地回避Sac，并保持回避3天。然而，当在注射LiCl之前注射亚催眠剂量的异丙酚时，Sac摄入量在第二个测试日突然增加，并且在调节后的第三个测试日几乎完全消失。CTA的消退过程几乎不受丙泊酚催眠剂量的影响。这些结果表明，异丙酚影响CTA记忆的保持机制，并呈剂量依赖性。亚催眠剂量异丙酚可能影响CTA记忆巩固的亚细胞过程。
Subhypnotic doses of propofol accelerate extinction of conditioned taste aversion. Some intravenous anesthetic agents including propofol is known to induce anterograde and retrograde amnesia. We evaluated whether propofol affect the long-term memory formed by the conditioned taste aversion (CTA) paradigm. Rats were allowed a 4h access to water through the experiments. After preconditioning water intake, the rats were offered 0.1% sodium saccharin (Sac) as conditioned stimulus (CS) for 20min. An intraperitoneal (i.p.) injection of several concentrations (0.5–100mg/kg) of propofol 10min after Sac exposure was followed by an i.p. injection of 0.15M LiCl (2% of body weight) as unconditioned stimulus (US) 30min after CS-exposure. The volumes of intake of Sac for 20min were measured on the successive 4 days. The rats, which acquired CTA by every CS–US paradigm, strongly avoided Sac on the first test day after conditioning and maintained the avoidance for 3 days. However, when subhypnotic dose of propofol was injected before LiCl-injection, Sac intake abruptly increased on the second test day and the almost complete extinction occurred on the third test day after conditioning. The extinction process of CTA was barely affected by hypnotic dose of propofol. These results suggest that propofol affects the retention mechanism of the CTA memory in a dose-dependent manner. Subhypnotic dose of propofol may affect the sub-cellular process of the memory consolidation in CTA.